Summary of Study ST001496
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001014. The data can be accessed directly via it's Project DOI: 10.21228/M8MT3W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001496 |
| Study Title | Application of developed quantatation method |
| Study Summary | To apply this method in a pilot study, we spiked either taurine or sulfoquinovose into an in vitro simplified human microbiota model with and without Bilophila wadsworthia, a known sulfonate utilizer. |
| Institute | Helmholtz Centre for Environmental Research |
| Last Name | Haange |
| First Name | Sven |
| Address | Permoserstrasse 1, Leipzig, Saxony, 04318, Germany |
| sven.haange@ufz.de | |
| Phone | 0049 341 2351099 |
| Submit Date | 2020-10-02 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-10-28 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001578 |
| Sampleprep Summary: | For the analysis of sulfonates by LC-MS/MS-MRM, aliquots of 250 µl for each sample were centrifuged (14 000 x g, 4 °C, 5 min) and 50 µl of the supernatant stored at – 20 °C until further processing. Samples were thawed, centrifuged at 18 000 x g at RT for 2 min and the supernatant was diluted 1:10 000 in 50% aqueous acetonitrile. Subsequently, 500 µl of each sample were placed in glass vials (Wicom, Heppenheim, Germany) and stored at - 80 °C until LC-MS/MS analysis. |
| Sampleprep Protocol Filename: | sven_haange_Sample_preparation-application_experiment.pdf |
