Summary of Study ST001638
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001035. The data can be accessed directly via it's Project DOI: 10.21228/M8X39Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001638 |
| Study Title | Metabolomics analysis of Vehicle (DMSO/PBS; (1:1) v/v) and DRB18 (10 mg/kg) treated A549 xenograft tumors |
| Study Type | Anticancer compound treatment experiment in vivo |
| Study Summary | A549 xenograft tumors were treated with (DMSO/PBS; (1:1) v/v) and DRB18 (10 mg/kg) for 5 weeks. The mice were then sacrificed and tumors were then excised. Tumors were then subjected to untargeted metabolomics analysis. |
| Institute | Ohio University |
| Department | Biological Sciences |
| Laboratory | Dr. Xiaozhuo Chen, Edison biotechnology Institute |
| Last Name | Shriwas |
| First Name | Pratik |
| Address | 172 Water Tower, Building 25, The Ridges, Konnekar Research Centerm Athens Ohio - 45701, USA |
| ps774614@ohio.edu | |
| Phone | 7406033801 |
| Submit Date | 2020-12-07 |
| Num Groups | 2 |
| Total Subjects | 20 |
| Num Males | 20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-04-01 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001721 |
| Sampleprep Summary: | Tumors were homogenized in a Beadbud homogenizer (Benchmark scientific) in a mixture of LC-MS grade ice-cold methanol and water (1:1; v/v). The supernatant was collected and sonicated in a water bath incubator for 15 minutes, followed by centrifugation at 13,000 rpm for 10 minutes and collection of the supernatant. Supernatants collected from in vitro and in vivo extraction were then lyophilized using a speed vacuum evaporator. The samples were then dissolved into a mixture of LC-MS grade acetonitrile/water (1:1; v/v) for analysis. |
| Sampleprep Protocol Filename: | prats1988_20201207_Invivo_collection_and_preparation_method.docx |
| Processing Method: | Metabolite extraction; Quenching; speed vacuum evaporation |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Quenching with ice cold methanol |
| Extract Enrichment: | Speed vaccum evaporator |
| Sample Resuspension: | Acetonitrile/waster (1:1) |
