Summary of Study ST001654

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001060. The data can be accessed directly via it's Project DOI: 10.21228/M8PH55 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001654
Study TitleMetabolic profiling of plasma collected from Peromyscus leucopus and Mus musculus following LPS treatment
Study TypeUntargeted Metabolomic profiling
Study SummarySmall molecule metabolites were extracted from plasma from Peromyscus leucopus and Mus musculus BALB/c and analyzed by liquid chromatography couple with mass spectrometry (LC-MS). XCMS software was used for peak picking, grouping and retention time correction from LC-MS data and generated a molecular feature spreadsheet. Pathway way enrichment analysis was carried out using Mummichog pathway analysis software using data from feature table.
Institute
Colorado State University
DepartmentMicrobiology, Immunology and Pathology
LaboratoryBelisle Lab
Last NameNurul
First NameIslam
Address3185 Rampart Road, Fort Collins, CO 80521
Emailnurul.islam@colostate.edu
Phone970 492 4249
Submit Date2021-01-13
Num Groups4
Total Subjects40
Num Males20
Num Females20
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-03-15
Release Version1
Islam Nurul Islam Nurul
https://dx.doi.org/10.21228/M8PH55
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001737
Sampleprep Summary:Metabolites were extracted from aliquots of 40 µL of plasma with 75% LC-MS grade methanol, supernatants were dried, resuspended in 50% methanol, 10 µL samples were subjected to liquid chromatography and mass spectrometry. Pooled quality control sample was prepared by mixing small aliquot (10 µL) of each plasma sample in the study.
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