Summary of Study ST001723
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001104. The data can be accessed directly via it's Project DOI: 10.21228/M80M6C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001723 |
Study Title | The effects of birth weight and breeding value for protein deposition on the plasma metabolome in growing pigs (part-II) |
Study Summary | An experiment was conducted with growing pigs, to determine the effects of birth weight (BiW) and estimated breeding value for protein deposition (EBV) on the metabolomic profile in plasma samples collected under different dietary regimens: protein adequate (A) or protein restricted (R, 70% of A). |
Institute | Aarhus University |
Department | Animal Science |
Laboratory | Metabolomics LC-MS platform Aarhus University Foulum |
Last Name | Hedemann |
First Name | Mette |
Address | Blichers Alle 20, Tjele, -, 8830, Denmark |
Mette.Hedemann@anis.au.dk | |
Phone | 51448783 |
Submit Date | 2021-03-14 |
Total Subjects | 40 |
Num Males | 40 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2021-03-31 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001806 |
Sampleprep Summary: | Plasma samples (150 µL) were pipetted into a 96-well plate and 450 µL acetonitrile containing an internal standard mix of glycocholic acid (glycine-1-13C) and p-chlorophenylalanine to a final concentration of 0.01 mg/mL, was added per well. The samples were mixed immediately and placed at 4°C for 10 min for protein precipitation. After centrifugation (3700 rpm for 25 min at 4°C), the supernatant was transferred to a filter plate fixed on top of a 96-well collection plate in a manifold with a pressure gauge. Vacuum was then applied to the filter plate, and the solvent containing plasma components was collected into the 96-well collection plate. The collection plate was placed in a vacuum centrifuge and the samples were evaporated to dryness (ca. 2.5 h, 805 × g and 30°C). A volume of 150 µL of water/acetonitrile/formic acid (95/5/0.1%) was added to the dried extracts in the collection plate. The dried collection plate was then sealed with a piece of aluminum laminate (#186002789, Waters), centrifuged (3700 rpm for 25 min at 4°C), and kept in the auto-sampler at 10°C for the UPLC-QTOF/MS analysis. The injection volume was 3 µl. |
Processing Storage Conditions: | -80℃ |
Extract Storage: | -80℃ |