Summary of Study ST001724
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001104. The data can be accessed directly via it's Project DOI: 10.21228/M80M6C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001724 |
Study Title | The effects of birth weight and breeding value for protein deposition on the urine metabolome in growing pigs (part-III) |
Study Summary | An experiment was conducted with growing pigs, to determine the effects of birth weight (BiW) and estimated breeding value for protein deposition (EBV) on the metabolomic profile in urine samples collected under different dietary regimens: protein adequate (A) or protein restricted (R, 70% of A). |
Institute | Aarhus University |
Department | Animal Science |
Laboratory | Metabolomics LC-MS platform Aarhus University Foulum |
Last Name | Hedemann |
First Name | Mette |
Address | Blichers Alle 20, Tjele, -, 8830, Denmark |
Mette.Hedemann@anis.au.dk | |
Phone | 51448783 |
Submit Date | 2021-03-15 |
Total Subjects | 40 |
Num Males | 40 |
Publications | The effects of birth weight and breeding value for protein deposition on nitrogen efficiency in growing pigs |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2021-03-31 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001807 |
Sampleprep Summary: | In a 96-well plate, the urine samples (112.5 µL) were diluted with H2O (112.5 µL) and 25 µL acetonitrile (100%, ACN) containing an internal standard mix of glycocholic acid (glycine-1-13C) and p-chlorophenylalanine to a final concentration of 0.01 mg/mL was added. The samples were incubated for 20 min at 4°C and subsequently centrifuged (3700 rpm, 25 min, 4°C). The supernatant, 100 µL per well, was transferred to a 200 µL 96-well plate and a protective film was welded on the plate using a heat sealer, and the plate was centrifuged at 3700 rpm, 4°C for 25 min prior to the LC-MS analysis. Samples were kept in the autosampler at 10°C, and the injection volume was 3 µl. |