Summary of Study ST001724

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001104. The data can be accessed directly via it's Project DOI: 10.21228/M80M6C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001724
Study TitleThe effects of birth weight and breeding value for protein deposition on the urine metabolome in growing pigs (part-III)
Study SummaryAn experiment was conducted with growing pigs, to determine the effects of birth weight (BiW) and estimated breeding value for protein deposition (EBV) on the metabolomic profile in urine samples collected under different dietary regimens: protein adequate (A) or protein restricted (R, 70% of A).
Institute
Aarhus University
DepartmentAnimal Science
LaboratoryMetabolomics LC-MS platform Aarhus University Foulum
Last NameHedemann
First NameMette
AddressBlichers Alle 20, Tjele, -, 8830, Denmark
EmailMette.Hedemann@anis.au.dk
Phone51448783
Submit Date2021-03-15
Total Subjects40
Num Males40
PublicationsThe effects of birth weight and breeding value for protein deposition on nitrogen efficiency in growing pigs
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2021-03-31
Release Version1
Mette Hedemann Mette Hedemann
https://dx.doi.org/10.21228/M80M6C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001807
Sampleprep Summary:In a 96-well plate, the urine samples (112.5 µL) were diluted with H2O (112.5 µL) and 25 µL acetonitrile (100%, ACN) containing an internal standard mix of glycocholic acid (glycine-1-13C) and p-chlorophenylalanine to a final concentration of 0.01 mg/mL was added. The samples were incubated for 20 min at 4°C and subsequently centrifuged (3700 rpm, 25 min, 4°C). The supernatant, 100 µL per well, was transferred to a 200 µL 96-well plate and a protective film was welded on the plate using a heat sealer, and the plate was centrifuged at 3700 rpm, 4°C for 25 min prior to the LC-MS analysis. Samples were kept in the autosampler at 10°C, and the injection volume was 3 µl.
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