Summary of study ST001820

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001150. The data can be accessed directly via it's Project DOI: 10.21228/M82690 This work is supported by NIH grant, U2C- DK119886.


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Study IDST001820
Study TitleWT neurons treated with APOE3/3 and APOE4/4 ACM
Study SummaryWe performed a targeted lipidomic analysis on WT neurons treated with astrocyte conditioned media (ACM) from APOE3/3 or APOE4/4 astrocytes.
Columbia University
Last NameNuriel
First NameTal
Address630 W 168th St., P&S 12-430
Submit Date2021-06-02
Analysis Type DetailLC-MS
Release Date2021-06-10
Release Version1
Tal Nuriel Tal Nuriel application/zip

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Sample Preparation:

Sampleprep ID:SP001903
Sampleprep Summary:Lipid and small-molecule metabolite extraction was performed using a methyl tert-butyl ether (MTBE)/methanol extraction protocol. Briefly, harvested neurons were homogenized in ice-cold methanol. Following homogenization, samples were incubated in 1200 ul of MTBE for 1 hr at room temperature to separate organic-soluble lipids from aqueous-soluble lipids and other small-molecules. Finally, 360 ul of ultrapure water was added (for a final ratio of 3:1:0.9 MTBE:methanol:water) to resolve the two liquid phases, and each samples were centrifuged at 10,000 x g for 10 min. For this experiment, the upper organic phase was collected from each sample and stored in a separate tube, and the remaining protein pellets were resuspended in 25 mM ammonium bicarbonate, pH 8, with 2.5% SDS. A BCA protein assay was performed on each protein fraction, and the organic phase was normalized to their protein concentration equivalent with 100% methanol. All samples were then stored at -80°C prior to analysis.