Summary of study ST001959

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001246. The data can be accessed directly via it's Project DOI: 10.21228/M8NM5W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001959
Study TitleInvestigation of serum metabolites in the AMPK intestinal KO mice
Study SummaryConducted serum untargeted metabolomics analysis in AMP-activated protein kinase (AMPK) intestinal KO mice and control mice under high-fat diet (HFD) conditions
Institute
City of Hope National Medical Center
DepartmentDiabetes Complications & Metabolism
Last NameZhang
First NameEryun
Address1500 Duarte Rd, Duarte, CA91010
Emailzhangey0220@126.com
Phone16268735517
Submit Date2021-10-27
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2022-01-08
Release Version1
Eryun Zhang Eryun Zhang
https://dx.doi.org/10.21228/M8NM5W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP002045
Sampleprep Summary:50 µL of serum was used for metabolites extraction. 200 μL extract solution (acetonitrile: methanol = 1: 1) containing isotopically labeled internal standard mixture was added to each sample. After vortexing for 1 min, the samples were sonicated for 10 min in cold water bath. Then, the samples were incubated at -40 °C for 1 h, and centrifuged at 12000 rpm for 15 min at 4°C. 200 µL of supernatant were transferred to a fresh tube, and dried in a vacuum concentrator at 37 °C. Then, the dried samples were reconstituted in 200 µL of 50% acetonitrile by sonication on ice for 10 min. The constitution was then centrifuged at 13000 rpm for 15 min at 4°C, and 75 µL of supernatant were transferred to a fresh glass vial for LC/MS analysis.
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