Summary of Study ST001959
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001246. The data can be accessed directly via it's Project DOI: 10.21228/M8NM5W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001959 |
Study Title | Investigation of serum metabolites in the AMPK intestinal KO mice |
Study Summary | Conducted serum untargeted metabolomics analysis in AMP-activated protein kinase (AMPK) intestinal KO mice and control mice under high-fat diet (HFD) conditions |
Institute | City of Hope National Medical Center |
Department | Diabetes Complications & Metabolism |
Last Name | Zhang |
First Name | Eryun |
Address | 1500 Duarte Rd, Duarte, CA91010 |
zhangey0220@126.com | |
Phone | 16268735517 |
Submit Date | 2021-10-27 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2022-01-08 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002045 |
Sampleprep Summary: | 50 µL of serum was used for metabolites extraction. 200 μL extract solution (acetonitrile: methanol = 1: 1) containing isotopically labeled internal standard mixture was added to each sample. After vortexing for 1 min, the samples were sonicated for 10 min in cold water bath. Then, the samples were incubated at -40 °C for 1 h, and centrifuged at 12000 rpm for 15 min at 4°C. 200 µL of supernatant were transferred to a fresh tube, and dried in a vacuum concentrator at 37 °C. Then, the dried samples were reconstituted in 200 µL of 50% acetonitrile by sonication on ice for 10 min. The constitution was then centrifuged at 13000 rpm for 15 min at 4°C, and 75 µL of supernatant were transferred to a fresh glass vial for LC/MS analysis. |