Summary of Study ST002112

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001338. The data can be accessed directly via it's Project DOI: 10.21228/M8RX2S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples | Perform analysis on untargeted data
Download mwTab file (text) | Download mwTab file(JSON) | Download data files (Contains raw data)

Study ID	ST002112
Study Title	Global, distinctive and personal changes in molecular and microbial profiles induced by specific fibers in humans (Untargeted)
Study Summary	Dietary fibers act through the microbiome and improve cardiovascular health, metabolic disorders and cancer prevention. To understand health benefits of dietary fiber supplementation we investigated two popular purified fibers, arabinoxylan (AX) and long-chain inulin (LCI), and a mixture of five fibers. We present multi-omic signatures of metabolomics, lipidomics, proteomics, metagenomics, a cytokine panel and clinical measurements on healthy and insulin resistant participants. Each fiber is associated with fiber-dependent biochemical and microbial responses. AX consumption associates with a significant reduction in LDL and an increase in bile acids, contributing to its observed cholesterol reduction. LCI is associated with an increase in Bifidobacterium. However, at the highest LCI dose there is increased inflammation and elevation in the liver enzyme alanine aminotransferase. This study yields insights into the effects of fiber supplementation, it provides insights into mechanisms behind fiber induced cholesterol reduction, and it shows effects of individual, purified fibers on the microbiome.
Institute	Stanford University
Last Name	Lancaster
First Name	Samuel
Address	240 Pasteur Dr, BMI bldg 4400, Stanford California, 94305
Email	slancast@stanford.edu
Phone	6126004033
Submit Date	2022-05-06
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2023-03-13
Release Version	1

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP002607
Sampleprep Summary:	We performed an untargeted metabolomics using a platform, previously described in Contrepois et al., 2015 (Contrepois et al., 2015) to profile plasma extracted metabolites. Samples were thawed and 100 μL of plasma was transferred into a 2mL Protein Lobind Eppendorf tube. In order to precipitate and remove proteins, 400 μL of 1:1:1 methanol:acetonitrile:acetone solution, including 17 internal standards to control for extraction efficiency and evaluate LC-MS performance, was added to each plasma sample while on a cold plate. Samples were placed on the vortex shaker for 15 minutes at 4C then placed at -20C for two hours to allow for sufficient protein precipitation. Samples were centrifuged at 10,000 rpm for 10 min at 4C and the supernatant (metabolite extract) from each sample was carefully removed and placed in a new 2mL Protein Lobind Eppendorf tube. Samples were placed in the turbovap, evaporated to dryness under nitrogen and stored at -80C. Before analysis on the MS, samples were reconstituted in 100 μL 1:1 methanol:water solution and vortexed for 30s, placed in a bath sonicator for 30s and incubated on ice for 30s. This process was repeated 3 times for all samples and were centrifuged at 10,000 rpm for 10 min at 4C. The supernatant of each of these was then transferred to MS tubes and stored at -20C until ran on the mass spectrometer.

Sampleprep ID:

SP002607

Sampleprep Summary:

We performed an untargeted metabolomics using a platform, previously described in Contrepois et al., 2015 (Contrepois et al., 2015) to profile plasma extracted metabolites. Samples were thawed and 100 μL of plasma was transferred into a 2mL Protein Lobind Eppendorf tube. In order to precipitate and remove proteins, 400 μL of 1:1:1 methanol:acetonitrile:acetone solution, including 17 internal standards to control for extraction efficiency and evaluate LC-MS performance, was added to each plasma sample while on a cold plate. Samples were placed on the vortex shaker for 15 minutes at 4C then placed at -20C for two hours to allow for sufficient protein precipitation. Samples were centrifuged at 10,000 rpm for 10 min at 4C and the supernatant (metabolite extract) from each sample was carefully removed and placed in a new 2mL Protein Lobind Eppendorf tube. Samples were placed in the turbovap, evaporated to dryness under nitrogen and stored at -80C. Before analysis on the MS, samples were reconstituted in 100 μL 1:1 methanol:water solution and vortexed for 30s, placed in a bath sonicator for 30s and incubated on ice for 30s. This process was repeated 3 times for all samples and were centrifuged at 10,000 rpm for 10 min at 4C. The supernatant of each of these was then transferred to MS tubes and stored at -20C until ran on the mass spectrometer.