Summary of Study ST002279
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001459. The data can be accessed directly via it's Project DOI: 10.21228/M84H7Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002279 |
| Study Title | Modulation of blood metabolites by dietary β–glucan in rainbow trout (Oncorhynchus mykiss) - Serum amino acids and biogenic amines concentrations |
| Study Summary | Measurement of changes in serum amino acids and biogenic amines levels using targeted mass spectrometry |
| Institute | Helmholtz Centre for Environmental Research |
| Last Name | Haange |
| First Name | Sven |
| Address | Permoserstrasse 1, Leipzig, Saxony, 04318, Germany |
| sven.haange@ufz.de | |
| Phone | 0049 341 2351099 |
| Submit Date | 2022-08-19 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-09-22 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002371 |
| Sampleprep Summary: | Sample preparation for MetIDQ p180 Kit measurement Solvents: Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS) Water MiliQ, Extracting agent - ACN / H2O (1:1) Equipment 4 steel balls size M Eppendorf Tubes 2mL Tissue slicer (Rettberg, Germany) Centrifuge (Sigma) Work steps Approximately 100 mg of sample and 4 steel balls of size M into each tube. Per mg of sample 5 µL of extracting agent was added. Shake the samples for 10 minutes at 30 Hz in the tissue slicer and centrifuge for 2 minutes at 14000 rpm. 10µL of supernatant was used for the targeted analytics. For blood sample analysis 10 µL plasma was taken. Kit reparation The analysis was performed using the validated MetIDQ p180 Kit and described in Siskos et al. [1]. Data processing was carried out with the provided quantitation method Kit (Biocrates Life Sciences AG, Innsbruck, Austria). 1 Siskos AP, Jain P, Römisch-Margl W, Bennett M, Achaintre D, Asad Y, et al. Interlaboratory Reproducibility of a Targeted Metabolomics Platform for Analysis of Human Serum and Plasma. Anal Chem 2017;89:656-65. |
| Sampleprep Protocol Filename: | Sample_preparation.pdf |
