Summary of Study ST002696
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001668. The data can be accessed directly via it's Project DOI: 10.21228/M83H7N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002696 |
| Study Title | ATP10A promotes endothelial cell insulin sensitivity and protects against female-specific dyslipidemia |
| Study Type | MS Untargeted Lipidomics |
| Study Summary | Endothelial dysfunction is strongly associated with metabolic and cardiovascular disease but how genetic and environmental factors intersect to promote disease progression remains elusive. Genetic association studies have linked ATP10A and closely related type IV P-type ATPases (P4-ATPases) to insulin resistance and vascular complications, such as atherosclerosis. Here, we generated Atp10A knockout mice and show that Atp10A deficiency results in female-specific dyslipidemia, independent of diet-induced obesity, characterized by elevated plasma triglycerides, free fatty acids and cholesterol, and altered HDL properties. We also observed increased circulating levels of several sphingolipid species and reduced levels of eicosanoids and bile acids. The Atp10A-/- mice also display hepatic insulin resistance without perturbations to whole-body glucose homeostasis. ATP10A is expressed in vascular and lymphatic endothelial cells, where reduced expression causes hyperactive insulin receptor signaling at basal insulin levels and an inability to properly respond to added insulin. This unique form of insulin resistance suggests that ATP10A promotes insulin receptor desensitization to maintain endothelial cell insulin sensitivity. These findings are clinically relevant because therapeutics that enhance ATP10A expression could improve vascular health and lipid metabolic profiles. |
| Institute | Vanderbilt University |
| Department | Chemistry |
| Laboratory | Center for Innovative Technology |
| Last Name | May |
| First Name | Jody |
| Address | 2301 Vanderbilt Place, Nashville, TN, 37235, USA |
| jody.c.may@vanderbilt.edu | |
| Phone | 615-875-8438 |
| Submit Date | 2023-04-20 |
| Num Groups | 2 |
| Total Subjects | 10 |
| Publications | submitted |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d, mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-05-24 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002804 |
| Sampleprep Summary: | 100 uL of plasma was collected from each mouse. An isotopically labeled lipid mixture (SPLASH LIPIDOMIX, Avanti) was added as an internal standard to each plasma sample. A liquid-liquid extraction was performed by adding 1 mL methyl tert-butyl ether (MTBE), vortexing, and subsequently centrifuging for 10 minutes at 10,000 rpm and 4˚C. The nonpolar, MTBE fraction (top liquid layer) containing the lipophilic components was removed and dried under vacuum centrifugation. Dried samples were stored at -80˚C until the day of MS analysis. For LC-IM-MS analysis, dried samples were resuspended in 100 µL IPA containing 40 µg/mL heptadecanoic acid and nonadecanoic acid, as well as 10 µg/mL glucosyl(β) sphingosine and N-heptadecanoyl-D-erythrosphingosine. |
| Processing Storage Conditions: | 4℃ |
| Extraction Method: | MTBE |
| Extract Enrichment: | vacuum centrifuge |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 100 uL IPA |
| Sample Derivatization: | n/a |
| Sample Spiking: | 14 heavy-labeled lipids prior to extraction; 4 odd-chain lipids during reconstitution |
| Subcellular Location: | n/a |
