Summary of Study ST003239

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002013. The data can be accessed directly via it's Project DOI: 10.21228/M8DJ7S This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003239
Study TitleLipidomic analysis of adipose from Gclc WT and whole-body Gclc KO mice.
Study SummaryWe found decreased triglycerides in the adipose of whole-body deletion of Gclc mice compared to wildtype mice.
Institute
University of Rochester Medical Center
Last NameHarris
First NameIsaac
Address601 Elmwood Ave, Rochester, New York, 14642-0001, USA
Emailisaac_harris@urmc.rochester.edu
Phone8572348624
Submit Date2024-05-29
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-06-10
Release Version1
Isaac Harris Isaac Harris
https://dx.doi.org/10.21228/M8DJ7S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP003365
Sampleprep Summary:Tissues were homogenized with a pre-chilled BioPulverizer (59012MS, BioSpec) and then placed on dry ice. The chloroform:methanol extraction solvent (v:v = 1:1) was added to the homogenate to meet 50 mg/mL. The samples were then sonicated in ice-cold water using BiorupterTM UCD-200 sonicator for 5 min (30 s sonication and 30 s rest cycle; high voltage mode). The lipid extracts were cleared by centrifugation (17,000g, 20°C, 10 min), and the lipids in the supernatant were analyzed by LC-MS.
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