Summary of Study ST003398
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002104. The data can be accessed directly via it's Project DOI: 10.21228/M8NJ9Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003398 |
| Study Title | Specific activation of the integrated stress response (ISR) uncovers regulation of lipid droplet biogenesis |
| Study Type | Biology |
| Study Summary | U2OS cells were treated with Dimerizer-PERK for 0h,1h,2h,4h,8h and 24h. Lipidomics analysis using LC-MS was performed on these samples to understand the regulation of cellular lipidome upon ISR activation |
| Institute | Calico Life Sciences |
| Department | Department of Mass Spectrometry-Technology Lab |
| Laboratory | Metabolomics Lab |
| Last Name | Vu |
| First Name | Ngoc |
| Address | 1130 Veterans BLVD, South San Francisco, CA 94080 |
| ngoc@calicolabs.com | |
| Phone | 650-420-5430 |
| Submit Date | 2024-08-08 |
| Num Groups | 6 |
| Total Subjects | 18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-09-03 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003530 |
| Sampleprep Summary: | Cells were cultured in 6-well plates, and ~500,000 cells per well were washed with warm PBS and quenched with 0.8 mL of 50/50 MeOH/H2O containing 100 µL of SPLASH LIPIDOMIXLipidSplash internal standards (Avanti Polar Lipids), followed by incubation at -20℃ for 15 minutes. Samples were scraped and collected to a 2 mL glass vial and extracted for lipidomics analysis using an MTBE-LLE extraction adopted from Matyash, et al 87. In brief, 800 μL of MTBE was added to the mixture, vortexed for 30 seconds and incubated on ice for another 15 minutes and centrifuged at 3500 RPM for 10 minutes at 4℃. Lipids partitioned in the top layer were collected into a separate vial, and the extraction process was repeated with an addition of 600 µL of MTBE. After incubating and centrifuging, the second organic layer was collected and combined in the first lipid vial, dried under nitrogen at 4℃, resuspended in 200 µL of ButOH/MeOH/H2O (2:1:1, v/v/v) for lipid analysis. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Matyash |
| Extract Storage: | 4℃ |
| Sample Resuspension: | ButOH/MeOH/H2O (2:1:1, v/v/v) |
