Summary of Study ST003400

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002106. The data can be accessed directly via it's Project DOI: 10.21228/M8D256 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST003400
Study Title	A Study on the Mechanism of Perinatal BPS Exposure Promoting Obesity Based on Metabolomics and Microbiomics
Study Summary	Due to increasingly stringent regulations on bisphenol A (BPA) usage, its structurally similar counterpart, BPS, has become widely employed as the primary substitute in various industries such as food packaging. However, recent years have unveiled potential risks of obesity promotion and insulin resistance associated with BPS, particularly during early life stages, although its precise impact remains inadequately understood. Addressing these concerns, this study established a mouse model to investigate the effects of maternal BPS exposure during pregnancy and lactation, combined with offspring consumption of a high-fat diet. The research examined physiological indicators related to obesity and insulin resistance in offspring, evaluated pathological changes in vital organs including the heart, liver, pancreas, white adipose tissue (WAT), and brown adipose tissue (BAT), conducted metabolomics perturbation analysis across multiple organs, and performed microbiome analysis based on fecal samples. Finally, correlations between phenotype, metabolome, and microbiome were explored to unravel intergenerational effects and mechanisms under BPS exposure, aiming to identify potential biomarkers of its effects.
Institute	College of Marine Food and Biological Engineering, Jimei University
Last Name	Li
First Name	Shuyin
Address	No.185 Yinjiang Road,Jimei District,Xiamen City,Fujian Province,China
Email	164000107@qq.com
Phone	18750682266
Submit Date	2024-08-02
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2024-09-03
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003532
Sampleprep Summary:	Each replicate of mouse (1 tissue, ~15 mg) was accurately weighted and transferred to an Eppendorf tube. Then, each sample was added 200 μL of Milli-Q water,followed by tissue homogenization using the bead-based homogenizer (Tissuelyser-24, Shanghai jingxin industrial development co., ltd, China) for 2 min at 65 Hz twice and the other samples including white adipose and brown adipose were homogenize for 2 min at 65 Hz four times. Next, each sample was added with 800 μL of precooled MeOH/ACN（1:1,v/v）containing standards (i.e., 0.49 μg/mL of Phenylalanine-d5(Phe-d5), 0.50 μg/mL of Tryptophan-d5(Trp-d5), 0.25 μg/mL of Carnitine C2-d3, 0.40 μg/mL of Cholic acid-d4(CA-d4), 0.24 μg/mL of Chenodeoxycholic acid-d4(CDCA-d4), 0.50 μg/mL of palmitic acid (fatty acid (FA) (16:0))-d3, and 0.5 μg/mL of stearic acid (FA(18:0))-d3)，the mixture was thoroughly vortexed for 30 s, use ultrasonic cleaning machine ice bath for 10 min, stand at -20 °C for 1 h, centrifugation at 15,000 g for 15 min at 4 ℃. The supernatant was collected and vacuum-dried in a refrigerated CentriVap concentrator (Labconco, USA), followed by storage at -80 °C until subsequent analysis.

Sampleprep ID:

SP003532

Sampleprep Summary:

Each replicate of mouse (1 tissue, ~15 mg) was accurately weighted and transferred to an Eppendorf tube. Then, each sample was added 200 μL of Milli-Q water,followed by tissue homogenization using the bead-based homogenizer (Tissuelyser-24, Shanghai jingxin industrial development co., ltd, China) for 2 min at 65 Hz twice and the other samples including white adipose and brown adipose were homogenize for 2 min at 65 Hz four times. Next, each sample was added with 800 μL of precooled MeOH/ACN（1:1,v/v）containing standards (i.e., 0.49 μg/mL of Phenylalanine-d5(Phe-d5), 0.50 μg/mL of Tryptophan-d5(Trp-d5), 0.25 μg/mL of Carnitine C2-d3, 0.40 μg/mL of Cholic acid-d4(CA-d4), 0.24 μg/mL of Chenodeoxycholic acid-d4(CDCA-d4), 0.50 μg/mL of palmitic acid (fatty acid (FA) (16:0))-d3, and 0.5 μg/mL of stearic acid (FA(18:0))-d3)，the mixture was thoroughly vortexed for 30 s, use ultrasonic cleaning machine ice bath for 10 min, stand at -20 °C for 1 h, centrifugation at 15,000 g for 15 min at 4 ℃. The supernatant was collected and vacuum-dried in a refrigerated CentriVap concentrator (Labconco, USA), followed by storage at -80 °C until subsequent analysis.