Summary of Study ST003554

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002188. The data can be accessed directly via it's Project DOI: 10.21228/M8SV5J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003554
Study Title	Investigation of age-dependent changes in the brain of honeybee workers using targeted metabolomics for amino acid and biogenic amine determination
Study Summary	In the honeybee guts we measured the concentrations of 21 amino acids and 6 biogenic amines. Using a k-means clustering, the metabolites were grouped by their trends into six clusters. Tyramine and tyrosine are involved in the octopamine and dopamine biosynthesis. We found that all four metabolites were part of the same cluster, with their concentrations being significantly decreased in comparison to newly emerged workers from day 1 onwards for the entire duration of the study.
Institute	Helmholtz Centre for Environmental Research
Department	Molecular Systems Biology
Last Name	Engelmann
First Name	Beatrice
Address	Permoserstraße 15, Leipzipg, Saxony, 03418, Germany
Email	beatrice.engelmann@ufz.de
Phone	004934160251099
Submit Date	2024-10-28
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2024-11-27
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003690
Sampleprep Summary:	In brief, x mg pool brain was mixed with five times the volume (in µL) of acetonitrile (ACN):Water (1:1, v/v) and homogenized using a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After Centrifugation (2 min, 14000 rpm), 10 µL were used for amino acid derivatization. First, the supernatant was evaporated to dryness (SpeedVac, Eppendorf), resuspended in 50 µL of 5% phenyl isothiocyanate (PITC) in ethanol: H2O: pyridine (1:1:1, v/v/v), and incubated for 25 min at RT. Subsequently, the samples were dried to remove excess PITC and resuspended in 10 µL 5 mM ammonium acetate in methanol. After incubation (10 min at 14,000 rpm) 90 µL of H2O: ACN + 0.2% formic acid were added. Each derivative was measured in an undiluted and diluted (1:25) manner.

Sampleprep ID:

SP003690

Sampleprep Summary:

In brief, x mg pool brain was mixed with five times the volume (in µL) of acetonitrile (ACN):Water (1:1, v/v) and homogenized using a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After Centrifugation (2 min, 14000 rpm), 10 µL were used for amino acid derivatization. First, the supernatant was evaporated to dryness (SpeedVac, Eppendorf), resuspended in 50 µL of 5% phenyl isothiocyanate (PITC) in ethanol: H2O: pyridine (1:1:1, v/v/v), and incubated for 25 min at RT. Subsequently, the samples were dried to remove excess PITC and resuspended in 10 µL 5 mM ammonium acetate in methanol. After incubation (10 min at 14,000 rpm) 90 µL of H2O: ACN + 0.2% formic acid were added. Each derivative was measured in an undiluted and diluted (1:25) manner.