Summary of Study ST004264
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002691. The data can be accessed directly via it's Project DOI: 10.21228/M8T837 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST004264 |
| Study Title | Dynamic metabolic and molecular changes during seasonal shrinking in Sorex araneus |
| Study Type | MS quantitative analysis |
| Study Summary | To meet the challenge of wintering in place many high-latitude small mammals reduce energy demands through hibernation. In contrast, short-lived Eurasian common shrews, Sorex araneus, remain active and shrink, including energy-intensive organs in winter, regrowing in spring in an evolved strategy called Dehnel’s phenomenon. How this size change is linked to metabolic and regulatory changes to sustain their high metabolism is unknown. We analyzed metabolic, proteomic, and gene expression profiles spanning the entirety of Dehnel’s seasonal cycle in wild shrews. We show regulatory changes to oxidative phosphorylation and increased fatty acid metabolism during autumn-to-winter shrinkage, as previously found in hibernating species. But in shrews we also found upregulated winter expression of genes involved in gluconeogenesis: the biosynthesis of glucose from non-carbohydrate substrates. Co-expression models revealed changes in size and metabolic gene expression interconnect via FOXO signaling, whose overexpression reduces size and extends lifespan in many model organisms. We propose that while shifts in gluconeogenesis meet the challenge posed by high metabolic rate and active winter lifestyle, FOXO signaling is central to Dehnel’s phenomenon, with spring downregulation limiting lifespan in these shrews. |
| Institute | Aalborg University |
| Department | Health Science and Technology |
| Laboratory | Molecular Pharmacology |
| Last Name | Zeng |
| First Name | Yuanyuan |
| Address | Thulevej 34 Aalborg SØ, Aalborg, Aalborg, 9210, Denmark |
| yuanyzeng@outlook.com | |
| Phone | +4555204123 |
| Submit Date | 2025-09-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-11-03 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP004423 |
| Sampleprep Summary: | Plasma was aliquoted and stored at −80 °C until extraction. For metabolite extraction, frozen aliquots were thawed on ice and proteins were precipitated by adding ice-cold organic solvent (methanol), followed by vortex mixing, brief incubation on ice, and centrifugation at high speed (4 °C). The clarified supernatant was transferred to new tubes and reconstituted in the initial LC–MS solvent prior to analysis. Blank and pooled QC extracts were prepared and processed alongside study samples under the same conditions. |
| Extract Storage: | -80℃ |
