Summary of Study ST002930

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001822. The data can be accessed directly via it's Project DOI: 10.21228/M86H8Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002930
Study TitleRole of Hypoxia-inducible factor-1a (HIF1a)in Skeletal Muscle Physiology (C2C12 myotube model)
Study SummaryHypoxia-inducible factor (HIF)-1a is continuously synthesized and degraded in normoxia, whereas during hypoxia, HIF1a stabilization restricts cellular oxygen utilization. Less is known about HIF1a function(s) and sex-specific effects during normoxia in the basal state. Since skeletal muscle is the largest protein store in mammals and protein homeostasis has high energy demands, we determined HIF1a function at baseline during normoxia in C2C12 murine myotubes with the use of untargeted metabolomics. 114 samples of extracted metabolites from cells were analyzed using LCMS. We identified that metabolites, especially those in the glycolytic pathway and TCA cycle, were differentially expressed in cells with HIF1a KO compared to WT.
Institute
Cleveland Clinic
DepartmentInflamation and Immunity
LaboratoryDasarathy Lab
Last NameDasarathy
First NameSrinivasan
Address9500 Euclid Avenue
Emaildasaras@ccf.org
Phone2163799846
Submit Date2023-10-05
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2024-10-28
Release Version1
Srinivasan Dasarathy Srinivasan Dasarathy
https://dx.doi.org/10.21228/M86H8Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Subject:

Subject ID:SU003043
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:Passage 4-8
Gender:Female
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