Summary of Study ST000095
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000087. The data can be accessed directly via it's Project DOI: 10.21228/M8F30Z This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000095 |
| Study Title | Dysfunctional lipid metabolism underlies the effect of perinatal DDT exposure on the development of metabolic syndrome |
| Study Type | Chemical dosage and feeding study |
| Study Summary | Targeted metabolomic analysis of bile acids was performed on 15 mouse liver samples collected from mice euthanized at 9 months following consumption of a high fat diet w/o perinatal DDT exposure. Funded by the National Institute of Health (R00 ES019919, R03 DK082724, U24 DK092993, U24 DK097154, T32 ES007059, and P60 DK020541), the American Diabetes Association, and USDA-ARS intramural Project 5306-51530-019-00D. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization. |
| Institute | University of California, Davis |
| Department | U.S.D.A. Western Human Nutrition Research Center |
| Laboratory | Newman |
| Last Name | Newman |
| First Name | John |
| Address | 430 W. Health Sciences Dr., Davis, CA 95616 |
| john.newman@ars.usda.gov | |
| Phone | +1-530-752-1009 |
| Submit Date | 2014-07-10 |
| Num Groups | 2 |
| Total Subjects | 15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Uploaded File Size | 23 M |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-02-03 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR000115 |
| Treatment Summary: | 1.7 mg DDT/kg body weight was administered to dams daily on gestational day 11 through postnatal day 5 and litters were culled to 6 mice at postnatal day 6. Mice were metabolically screened from 2-6 months when they consumed a high fat diet. Mice were sacrificed at 9 months of age. |
| Treatment Protocol Filename: | Newman_Lab_Bile_Acid_Protocols_La_Merrill_WCMC_P&F.pdf |
| Treatment: | Intervention |
| Treatment Compound: | p,p`-DDT, o,p`-DDT |
| Treatment Route: | Oral |
| Treatment Dose: | 77.2% p,p`-DDT (98.5% purity neat, AccuStandard, New Haven, CT) and 22.8% o,p`-DDT (100% purity neat, AccuStandard) were dissolved at a final concentration of 0.17 g DDT mixture/L organic olive oil |
| Treatment Doseduration: | From 11.5 days post coitus to postnatal day 5 |
| Treatment Vehicle: | Olive oil |
| Animal Endp Euthanasia: | 9 months |
| Animal Endp Tissue Coll List: | Liver |
| Animal Endp Tissue Proc Method: | Dissected, placed on dry ice |
