Summary of Study ST000307
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000247. The data can be accessed directly via it's Project DOI: 10.21228/M8VG6R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000307 |
| Study Title | Targeting CRBN to alter CD8 T cell metabolism |
| Study Type | T cell activation |
| Study Summary | The purpose of this study was to evaluate the metabolic profile of different treatments of T-Cells among two separate mouse genotypes. |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | Moffitt Cancer Center - Immunology |
| Last Name | Swearingen |
| First Name | Rebecca |
| Address | - |
| rebecca.swearingen@moffitt.org | |
| Phone | - |
| Submit Date | 2015-12-22 |
| Num Groups | 12 |
| Total Subjects | 60 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR000341 |
| Treatment Summary: | - |
| Animal Anesthesia: | CO2 asphyxiation |
| Animal Acclimation Duration: | Mice from Jackson are subjected to a week-long acclimation period in our facility prior to experimental use. |
| Animal Endp Tissue Coll List: | Spleen into cell culture media |
| Cell Growth Container: | 96-well round-bottom plate that was coated with anti-CD3 antibody |
| Cell Growth Rate: | Murine T cells generally divide after 48-72 hours of stimulation. |
| Cell Media: | RPMI 1640 with 10% FBS, pen/strep, gentamycin, NEAA, Sodium pyruvate, fungizone (Amphotericin B), 2-mercaptoethanol |
| Cell Envir Cond: | 37°C, normoxic conditions |
