Summary of Study ST000955

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000656. The data can be accessed directly via it's Project DOI: 10.21228/M8W39D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000955
Study TitleEffects of ODC inhibition on T cell metabolism
Study Typedrug treatment at two time
Study SummaryIsolated T cells were activated for 48-72 hours in the presence and absence of 5mM DFMO, an ODC inhibitor
Institute
University of Florida
DepartmentSECIM
Last NameHesterberg
First NameRebecca
Address12902 Magnolia Dr
Emailrebecca.hesterberg@moffitt.org
Phone813-270-9181
Submit Date2018-04-14
Num Groups4
Total Subjects16
Study CommentsOT-1 mice express a transgenic T cell receptor in all CD8+ T cells that binds with high affinity to a peptide derived from the protein ovalbumin (SIINFEKL).
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2019-05-15
Release Version1
Rebecca Hesterberg Rebecca Hesterberg
https://dx.doi.org/10.21228/M8W39D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR001008
Treatment Summary:Splenic CD8+ T cells were isolated from 4 OT-1 mice (WT1, 3, 7, 8) by negative immunomagnetic selection. T cells stimulated with ova-derived peptide (SIINFEKL) that binds with high affinity to the OT-1 transgenic TCR and 10ng/mL IL-2 with and without 5mM DFMO for 48-72 hours. Cell stoarge: 37 degrees, Cell growth container: 24 well plate, cell media: RPMI1640 with glutamine. Added components were: NEAA, Sodium Pyruvate, Fetal Bovine Serum, Fungizone, Gentimycin, Pen/strep.
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