Summary of Study ST000964

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000662. The data can be accessed directly via it's Project DOI: 10.21228/M83Q2T This work is supported by NIH grant, U2C- DK119886.


Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000964
Study TitleU13C Glucose Tracing of Young and Old Pancreatic Islets
Study SummaryPancreatic islets from young and old mice were traced with either 2.8mM or 16.8mM U13C glucose to determine the effect of age upon glucose metabolism in basal and stimulated states.
University of California, San Diego
Last NameWortham
First NameMatthew
Address2880 Torrey Pines Scenic Drive, Sanford Consortium for Regenerative Medicine, Room 3102
Submit Date2018-04-27
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2019-04-27
Release Version1
Matthew Wortham Matthew Wortham application/zip

Select appropriate tab below to view additional metadata details:


Treatment ID:TR001017
Treatment Summary:Islets were starved for 1 hour in KRBH supplemented with 2.8 mM glucose as for GSIS assays. Islets were then transferred to petri dishes containing U-13C-labeled glucose as indicated and immediately seeded into wells of non-treated 12-well plates at a density of 100 size-matched islets per replicate in a final volume of 500 μl KRBH containing basal (2.8 mM) or stimulatory (16.8 mM) concentrations of labeled glucose. Tracing was performed for the indicated durations at 37oC with 5% CO2. Following the trace, islets were rapidly chilled by swirling plate to resuspend islets then transferring entire contents of each well to an Eppendorf tube on ice. Islets were then pelleted by centrifugation at 500 x g for 2 min at 4oC, washed in 1 mL of ice cold 0.9% NaCl, centrifuged as before, then pellets were frozen in a dry ice/ethanol bath and stored at -80oC.