Summary of Study ST001023

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000684. The data can be accessed directly via it's Project DOI: 10.21228/M8868G This work is supported by NIH grant, U2C- DK119886.

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Study IDST001023
Study TitleH3K27M cells and glutamine metabolomics 1 million cell test (part-I)
Study SummaryTesting TCA concentrations of Diffuse Intrinsic Pontine Gliomas (DIPG) cellines with H3K27M mutations. One million cells are tested with a TCA concentrations panel. We are a high volume center for treating malignant gliomas, which gives us an advantage in obtaining tissue for these relatively rare tumors. We have developed several DIPG patient derived cell lines and xenografts that bear all the key molecular features of this disease including the H3K27M mutation and global H3K27 hypomethylation. These cells are low in passage and we think these lines more closely resemble the patients tumor pathology then established cell lines that have been in culture/mice for numerous years.
Institute
Mayo Clinic
Last NameDaniels
First NameDavid
Address200 First Street SW Rochester, MN 55905
Emaildaniels.david@mayo.edu
Phone507-284-2511
Submit Date2018-07-18
Analysis Type DetailGC-MS
Release Date2020-07-15
Release Version1
David Daniels David Daniels
https://dx.doi.org/10.21228/M8868G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR001076
Treatment Summary:Metabolic profiling will be conducted similarly to published methods and standard methods from our metabolic core. Glucose, glutamine and lactate levels in culture medium will be measured using gas chromatography/mass spectroscopy (GC/MS). Briefly, cells will be seeded in 6 well plates in triplicate using our standard neurosphere media (MH+++) and cultured for 24 to 48h before experiments. Changes in metabolite concentrations relative to fresh media will be normalized to protein content of each well. Cellular metabolite levels will be measured using standard protocols using deuterated 2-hydroxyglutarate (d5-5HG) as an internal standard and analyzed using GC/MS. To determine the fraction of metabolites from Glu and Gln, 13C versions of each metabolite [U-13C]glucose or [U-13C]glutamine (Cambridge Isotope Lab) will be used. All of these experiements will be performed by the Mayo Clinic Metabolomics Research Core.
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