Summary of Study ST001149
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000768. The data can be accessed directly via it's Project DOI: 10.21228/M8DH49 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001149 |
| Study Title | Plasmodium Niemann-Pick Type C1-Related Protein is a Druggable Target Required for Parasite Membrane Homeostasis |
| Study Type | Drug Exposure |
| Study Summary | Metabolic examination/confirmation of resistance mutations and GFP tagging observed in study |
| Institute | Pennsylvania State University |
| Department | BMB |
| Laboratory | Llinás Laboratory |
| Last Name | Llinás |
| First Name | Manuel |
| Address | W-122 Millennium Science Complex, Pollock Road |
| eso5005@psu.edu | |
| Phone | 8148673527 |
| Submit Date | 2019-02-15 |
| Raw Data Available | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-05-15 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR001229 |
| Treatment Summary: | Infected Red Blood cells were exposed to Antimalarial Compounds, associated with sample names. All samples were treated with 10xIC50 of a compound for 2.5 hours or left untreated for 2.5 hours. |
| Treatment Protocol ID: | Metabolite_Extraction_for_LCMS_2017_Llinas |
| Treatment Protocol Filename: | Metabolite_Extraction_for_LCMS_2017_Llinas.pdf |
| Treatment: | Abiotic |
| Treatment Compound: | ATQ, MMV009108, MMV019662, MMV028038 |
| Treatment Route: | Addition to media |
| Treatment Dose: | 10xIC50, 2-10xIC50 for archival data |
| Treatment Dosevolume: | Always equal to or less than 10 uL per 5 mL sample |
| Treatment Doseduration: | 2.5 hours |
| Treatment Vehicle: | Stocks kept in either DMSO or Water |
| Cell Storage: | Temperature/Gas Mixture controlled incubator |
| Cell Growth Container: | 6-well plate |
| Cell Growth Config: | 5 mL per well, each with 1x10^8 cells |
| Cell Media: | RPMI 1640 w/ Albumax, Gentamycin, Hypoxanthine, HEPES, Sodium Bicarbonate |
| Cell Harvesting: | PBS Wash followed by 90% methanol extraction |
