Summary of study ST001260

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000846. The data can be accessed directly via it's Project DOI: 10.21228/M8B97R This work is supported by NIH grant, U2C- DK119886.

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Study IDST001260
Study TitleMetabolic changes of Fusobacterium nucleatum when co-cultured with other oral microbes (part-I)
Study SummaryWe used membrane-separated co-culture systems to globally assess metabolomic changes of Fusobacterium nucleatum when co-cultured with Streptococcus gordonii and/or Veillonella parvula.
Institute
Osaka University
DepartmentGraduate School of Dentistry, Department of Preventive Dentistry
Last NameKuboniwa
First NameMasae
AddressYamadaoka 1-8
Emailkuboniwa@dent.osaka-u.ac.jp
Phone81668792922
Submit Date2019-09-26
Analysis Type DetailLC-MS
Release Date2021-04-01
Release Version1
Masae Kuboniwa Masae Kuboniwa
https://dx.doi.org/10.21228/M8B97R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR001343
Treatment Summary:Co-culture growth was performed by inoculating 1.4E+10 cells of F. nucleatum in CDM in the lower chamber of a Transwell unit with 0.4-µm pore polystyrene membrane inserts (Corning, NY, USA), into which 1.4E+10 cells of S. gordonii, V. parvula or their mixture (7E+9 cells each) in CDM, or an equal volume of CDM (as a control) were added. The setup was anaerobically incubated in triplicate for 37°C.
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