Summary of Study ST001260

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000846. The data can be accessed directly via it's Project DOI: 10.21228/M8B97R This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001260
Study Title	Metabolic changes of Fusobacterium nucleatum when co-cultured with other oral microbes (part-I)
Study Summary	We used membrane-separated co-culture systems to globally assess metabolomic changes of Fusobacterium nucleatum when co-cultured with Streptococcus gordonii and/or Veillonella parvula.
Institute	Osaka University
Department	Graduate School of Dentistry, Department of Preventive Dentistry
Last Name	Kuboniwa
First Name	Masae
Address	Yamadaoka 1-8
Email	kuboniwa@dent.osaka-u.ac.jp
Phone	81668792922
Submit Date	2019-09-26
Analysis Type Detail	LC-MS
Release Date	2021-04-01
Release Version	1

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Treatment:

Treatment ID:	TR001343
Treatment Summary:	Co-culture growth was performed by inoculating 1.4E+10 cells of F. nucleatum in CDM in the lower chamber of a Transwell unit with 0.4-µm pore polystyrene membrane inserts (Corning, NY, USA), into which 1.4E+10 cells of S. gordonii, V. parvula or their mixture (7E+9 cells each) in CDM, or an equal volume of CDM (as a control) were added. The setup was anaerobically incubated in triplicate for 37°C.

Treatment ID:

TR001343

Treatment Summary:

Co-culture growth was performed by inoculating 1.4E+10 cells of F. nucleatum in CDM in the lower chamber of a Transwell unit with 0.4-µm pore polystyrene membrane inserts (Corning, NY, USA), into which 1.4E+10 cells of S. gordonii, V. parvula or their mixture (7E+9 cells each) in CDM, or an equal volume of CDM (as a control) were added. The setup was anaerobically incubated in triplicate for 37°C.