Summary of Study ST001654
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001060. The data can be accessed directly via it's Project DOI: 10.21228/M8PH55 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001654 |
Study Title | Metabolic profiling of plasma collected from Peromyscus leucopus and Mus musculus following LPS treatment |
Study Type | Untargeted Metabolomic profiling |
Study Summary | Small molecule metabolites were extracted from plasma from Peromyscus leucopus and Mus musculus BALB/c and analyzed by liquid chromatography couple with mass spectrometry (LC-MS). XCMS software was used for peak picking, grouping and retention time correction from LC-MS data and generated a molecular feature spreadsheet. Pathway way enrichment analysis was carried out using Mummichog pathway analysis software using data from feature table. |
Institute | Colorado State University |
Department | Microbiology, Immunology and Pathology |
Laboratory | Belisle Lab |
Last Name | Nurul |
First Name | Islam |
Address | 3185 Rampart Road, Fort Collins, CO 80521 |
nurul.islam@colostate.edu | |
Phone | 970 492 4249 |
Submit Date | 2021-01-13 |
Num Groups | 4 |
Total Subjects | 40 |
Num Males | 20 |
Num Females | 20 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2021-03-15 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR001744 |
Treatment Summary: | Animals were anesthetized with isoflurane and injected i.p. with a single dose of E. coli O111:B4 LPS at a concentration of 10 µg/gm body weight in a 50 µl volume as described above. The control group was anesthetized and then injected with the 0.9% saline alone. The experiment started at 0800 h with 10 min intervals between animals and with alteration of LPS and control injections. At 4.0 hr after their injection the animals were euthanized. After opening the chest, exsanguination was performed by cardiac puncture and blood was transferred to a heparin sulfate coated tubes (Becton-Dickinson Microtainer). Anticoagulated blood was centrifuged to pellet blood cells for 3 min at 4600 x g at 4° C. Plasma and blood pellet was kept separately at -80° C until further analysis. |
Treatment Compound: | E. coli O111:B4 LPS |
Treatment Route: | i.p. |
Treatment Dose: | 10 µg/gm body weight |
Treatment Dosevolume: | 50 µl volume |
Treatment Vehicle: | sterile, endotoxin-free 0.9% saline |
Animal Anesthesia: | isoflurane |