Summary of Study ST003398

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002104. The data can be accessed directly via it's Project DOI: 10.21228/M8NJ9Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003398
Study TitleSpecific activation of the integrated stress response (ISR) uncovers regulation of lipid droplet biogenesis
Study TypeBiology
Study SummaryU2OS cells were treated with Dimerizer-PERK for 0h,1h,2h,4h,8h and 24h. Lipidomics analysis using LC-MS was performed on these samples to understand the regulation of cellular lipidome upon ISR activation
Institute
Calico Life Sciences
DepartmentDepartment of Mass Spectrometry-Technology Lab
LaboratoryMetabolomics Lab
Last NameVu
First NameNgoc
Address1130 Veterans BLVD, South San Francisco, CA 94080
Emailngoc@calicolabs.com
Phone650-420-5430
Submit Date2024-08-08
Num Groups6
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-09-03
Release Version1
Ngoc Vu Ngoc Vu
https://dx.doi.org/10.21228/M8NJ9Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR003532
Treatment Summary:U2OS cells were treated with 0.05nM of dimerizer over the course of 0,1,2,4,8,24 hours for lipidomics analysis
Treatment Compound:DImerizer AP20187
Treatment Dose:0.05 nM to media
Treatment Doseduration:0h,1h,2h,4h,8h,24h
Treatment Vehicle:ethanol
Cell Growth Container:6-well plate
Cell Growth Config:U2OS cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Sigma) and 1X antibiotic-antimycotic solution (Gibco). Cells were housed in an incubator at 37°C, 5% CO2, 20% O2 and passaged every 2-3 days with trypsin.
Cell Media:U2OS cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Sigma) and 1X antibiotic-antimycotic solution (Gibco).
Cell Harvesting:LLE-MTBE (Matyash)
Cell Pct Confluence:80%
Cell Media Lastchanged:24 hours prior to harvesting
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