Summary of Study ST003400

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002106. The data can be accessed directly via it's Project DOI: 10.21228/M8D256 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST003400
Study Title	A Study on the Mechanism of Perinatal BPS Exposure Promoting Obesity Based on Metabolomics and Microbiomics
Study Summary	Due to increasingly stringent regulations on bisphenol A (BPA) usage, its structurally similar counterpart, BPS, has become widely employed as the primary substitute in various industries such as food packaging. However, recent years have unveiled potential risks of obesity promotion and insulin resistance associated with BPS, particularly during early life stages, although its precise impact remains inadequately understood. Addressing these concerns, this study established a mouse model to investigate the effects of maternal BPS exposure during pregnancy and lactation, combined with offspring consumption of a high-fat diet. The research examined physiological indicators related to obesity and insulin resistance in offspring, evaluated pathological changes in vital organs including the heart, liver, pancreas, white adipose tissue (WAT), and brown adipose tissue (BAT), conducted metabolomics perturbation analysis across multiple organs, and performed microbiome analysis based on fecal samples. Finally, correlations between phenotype, metabolome, and microbiome were explored to unravel intergenerational effects and mechanisms under BPS exposure, aiming to identify potential biomarkers of its effects.
Institute	College of Marine Food and Biological Engineering, Jimei University
Last Name	Li
First Name	Shuyin
Address	No.185 Yinjiang Road,Jimei District,Xiamen City,Fujian Province,China
Email	164000107@qq.com
Phone	18750682266
Submit Date	2024-08-02
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2024-09-03
Release Version	1

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Treatment:

Treatment ID:	TR003534
Treatment Summary:	Briefly,C57BL/6 pregnant mice were fed ordinary diet under constant temperature [(23±1) ℃] and constant humidity [(55±5)%], and followed a strict 12 h/12 h light/dark cycle.Animals were kept in polystyrene rat cages for the experiment. They were divided into normal saline solvent as control, low-dose BPS group (0.05 mg/kg/d) and high-dose BPS group (5 mg/kg/d). BPS exposure was performed by subcutaneous injection. The general state of pregnant mice was observed daily, and their body mass was weighed once a week. After delivery, pregnant rats were exposed to BPS in different groups during lactation period, and the mothers were killed after lactation period. After lactation, F1 mice were observed in general state every day and weighed once a week. After 3 weeks of breastfeeding, F1 mice were fed adaptive diet for 1 week, followed by high-fat diet for 8 weeks. Mouse feces were collected, and mouse heart, liver, pancreas, white adipose and brown adipose were collected. After sampling, the mice were quickly washed in normal saline. After cleaning, the mice were treated with liquid nitrogen and finally frozen in the refrigerator at -80 ℃.

Treatment ID:

TR003534

Treatment Summary:

Briefly,C57BL/6 pregnant mice were fed ordinary diet under constant temperature [(23±1) ℃] and constant humidity [(55±5)%], and followed a strict 12 h/12 h light/dark cycle.Animals were kept in polystyrene rat cages for the experiment. They were divided into normal saline solvent as control, low-dose BPS group (0.05 mg/kg/d) and high-dose BPS group (5 mg/kg/d). BPS exposure was performed by subcutaneous injection. The general state of pregnant mice was observed daily, and their body mass was weighed once a week. After delivery, pregnant rats were exposed to BPS in different groups during lactation period, and the mothers were killed after lactation period. After lactation, F1 mice were observed in general state every day and weighed once a week. After 3 weeks of breastfeeding, F1 mice were fed adaptive diet for 1 week, followed by high-fat diet for 8 weeks. Mouse feces were collected, and mouse heart, liver, pancreas, white adipose and brown adipose were collected. After sampling, the mice were quickly washed in normal saline. After cleaning, the mice were treated with liquid nitrogen and finally frozen in the refrigerator at -80 ℃.