Summary of Study ST000017

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000016. The data can be accessed directly via it's Project DOI: 10.21228/M86P45 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000017
Study TitleRat HCR/LCR Stamina Study
Study TypeLC-MS analysis (Untargeted)
Study SummaryMaximal exercise-associated oxidative capacity is strongly correlated with health and longevity in humans. Rats selectively bred for high running capacity (HCR) have improved metabolic health and are longer-lived than their low-capacity counterparts (LCR). Using metabolomic and proteomic profiling, we show that HCR efficiently oxidize fatty acids (FAs) and branched-chain amino acids (BCAAs), sparing glycogen and reducing accumulation of short- and medium-chain acylcarnitines. HCR mitochondria have reduced acetylation of mitochondrial proteins within oxidative pathways at rest, and there is rapid protein deacetylation with exercise, which is greater in HCR than LCR. Fluxomic analysis of valine degradation with exercise demonstrates a functional role of differential protein acetylation in HCR and LCR. Our data suggest that efficient FA and BCAA utilization contribute to high intrinsic exercise capacity and the health and longevity benefits associated with enhanced fitness. Research is published, untargeted data not used in publication but project description is relevant: http://www.sciencedirect.com/science/article/pii/S1550413115000595
Institute
University of Michigan
DepartmentInternal Medicine
LaboratoryBurant Lab (MMOC)
Last NameQi
First NameNathan
Emailnathanqi@med.umich.edu
Phone734-232-0815
Submit Date2013-09-24
Num Groups2
Total Subjects42
Raw Data AvailableYes
Uploaded File Size6.8 M
Analysis Type DetailLC-MS
Release Date2013-10-24
Release Version1
Nathan Qi Nathan Qi
https://dx.doi.org/10.21228/M86P45
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000016
Project DOI:doi: 10.21228/M86P45
Project Title:Rat Stamina Studies
Project Type:Feeding
Project Summary:Stamina in rats
Institute:University of Michigan
Department:Internal Medicine
Laboratory:Burant Lab
Last Name:Beecher
First Name:Chris
Email:chrisbee@med.umich.edu
Phone:734-232-0815
Funding Source:NIH: R01 DK077200

Subject:

Subject ID:SU000033
Subject Type:Animal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Genotype Strain:Low Capacity Running | High Capacity Running
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Feeeding Running Capacity
SA001039S00009481Ad lib High
SA001040S00009480Ad lib High
SA001041S00009503Ad lib High
SA001042S00009502Ad lib High
SA001043S00009500Ad lib High
SA001044S00009501Ad lib High
SA001045S00009478Ad lib High
SA001046S00009479Ad lib High
SA001047S00009477Ad lib High
SA001060S00009471Ad lib Low
SA001061S00009472Ad lib Low
SA001062S00009474Ad lib Low
SA001063S00009475Ad lib Low
SA001064S00009473Ad lib Low
SA001065S00009499Ad lib Low
SA001066S00009494Ad lib Low
SA001067S00009470Ad lib Low
SA001068S00009495Ad lib Low
SA001069S00009496Ad lib Low
SA001070S00009498Ad lib Low
SA001071S00009497Ad lib Low
SA001048S00009513Calorie restricted High
SA001049S00009514Calorie restricted High
SA001050S00009492Calorie restricted High
SA001051S00009512Calorie restricted High
SA001052S00009511Calorie restricted High
SA001053S00009510Calorie restricted High
SA001054S00009509Calorie restricted High
SA001055S00009491Calorie restricted High
SA001056S00009493Calorie restricted High
SA001057S00009490Calorie restricted High
SA001058S00009489Calorie restricted High
SA001059S00009488Calorie restricted High
SA001072S00009482Calorie restricted Low
SA001073S00009508Calorie restricted Low
SA001074S00009484Calorie restricted Low
SA001075S00009483Calorie restricted Low
SA001076S00009486Calorie restricted Low
SA001077S00009504Calorie restricted Low
SA001078S00009505Calorie restricted Low
SA001079S00009506Calorie restricted Low
SA001080S00009507Calorie restricted Low
Showing results 1 to 42 of 42

Collection:

Collection ID:CO000017
Collection Summary:-
Sample Type:Serum
Volumeoramount Collected:50 ul

Treatment:

Treatment ID:TR000035
Treatment Summary:ad lib feeding | Calorie restricted feeding

Sample Preparation:

Sampleprep ID:SP000030
Sampleprep Summary:-
Sampleprep Protocol Filename:EX00166-Sample_preparation_protocol.pdf

Combined analysis:

Analysis ID AN000034 AN000035
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1200 Agilent 1200
Column Waters Acquity HSS T3 reversed-phase Waters Acquity HSS T3 reversed-phase
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000017
Chromatography Summary:Positive ion detection
Methods Filename:EX00166-LCMS-method.pdf
Instrument Name:Agilent 1200
Column Name:Waters Acquity HSS T3 reversed-phase
Column Temperature:40C
Flow Gradient:0-0.5 min 1% B, 0.5-2 min 1-99% B, 2-6 min 99% B, 6-9 min 1% B.
Flow Rate:0.35 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH000018
Chromatography Summary:Negative ion detection
Methods Filename:EX00166-LCMS-method.pdf
Instrument Name:Agilent 1200
Column Name:Waters Acquity HSS T3 reversed-phase
Column Temperature:40C
Flow Gradient:0-0.5 min 1% B, 0.5-2 min 1-99% B, 2-6 min 99% B, 6-9 min 1% B.
Flow Rate:0.35 mL/min
Solvent A:100% water; 0.1% ammonium bicarbonate
Solvent B:100% methanol; 0.1% ammonium bicarbonate
Chromatography Type:Reversed phase

MS:

MS ID:MS000033
Analysis ID:AN000034
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Reference masses were 99.09 and 922.009 m/z.
Ion Mode:POSITIVE
Capillary Voltage:3500V
Ion Source Temperature:350 C
Acquisition Parameters File:EX00166-LCMS-method.pdf
Processing Parameters File:EX00166-Data_analysis-method.pdf
  
MS ID:MS000034
Analysis ID:AN000035
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Reference masses 62.0009 and 981.9956 m/z.
Ion Mode:NEGATIVE
Capillary Voltage:3500V
Ion Source Temperature:350 C
Acquisition Parameters File:EX00166-LCMS-method.pdf
Processing Parameters File:EX00166-Data_analysis-method.pdf
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