Summary of Study ST000098
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000090. The data can be accessed directly via it's Project DOI: 10.21228/M85K56 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000098 |
| Study Title | Heatshock response of C. elegans using IROA (II) |
| Institute | University of Florida |
| Department | Department of Biochemistry and Molecular Biology and Southeastern Center for Integrated Metabolomics (SECIM) |
| Laboratory | Arthur Edison |
| Last Name | Edison |
| First Name | Arthur |
| Address | R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
| aedison@ufl.edu | |
| Phone | 352-392-4535 |
| Submit Date | 2014-09-15 |
| Num Groups | 1 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Uploaded File Size | 978 M |
| Analysis Type Detail | NMR |
| Release Date | 2015-11-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000090 |
| Project DOI: | doi: 10.21228/M85K56 |
| Project Title: | 13C Metabolomics: NMR and IROA for Unknown Identification |
| Project Summary: | Demonstrate a method using high-sensitivity NMR to identify an unknown metabolite from a fraction isolated from an IROA LC-MS experiment. |
| Institute: | University of Florida |
| Department: | Department of Biochemistry and Molecular Biology and Southeastern Center for Integrated Metabolomics (SECIM) |
| Laboratory: | Edison |
| Last Name: | Edison |
| First Name: | Arthur |
| Email: | aedison@ufl.edu |
| Phone: | 352-392-4535 |
Subject:
| Subject ID: | SU000117 |
| Subject Type: | Invertebrate |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
| Genotype Strain: | N2 |
| Species Group: | Invertebrate |
Factors:
Subject type: Invertebrate; Subject species: Caenorhabditis elegans (Factor headings shown in green)
| mb_sample_id | local_sample_id | Fractionation |
|---|---|---|
| SA005497 | HSP_F9_ms2_neg.raw | fraction 4-4.5 min |
| SA005498 | HSP_F9_pos.raw | fraction 4-4.5 min |
| SA005495 | HSP_F10_pos.raw | fraction 4.5-5 min |
| SA005496 | HSP_F10_ms2_neg.raw | fraction 4.5-5 min |
| SA005499 | HSP_ms2_pos.raw | Unfractionated |
| SA005500 | HSP_pos.raw | Unfractionated |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO000100 |
| Collection Summary: | Samples were already collected. |
| Collection Protocol Filename: | NMR_Methods.docx LCMSMethod.docx |
| Sample Type: | Worms |
Treatment:
| Treatment ID: | TR000118 |
| Treatment Summary: | No further treatment |
Sample Preparation:
| Sampleprep ID: | SP000113 |
| Sampleprep Summary: | Samples were mixed together and resuspended in 120 ?L of water. |
| Sampleprep Protocol Filename: | NMR_Methods.docx LCMSMethod.docx |
Analysis:
| Analysis ID: | AN000160 |
| Laboratory Name: | Arthur Edison |
| Analysis Type: | NMR |
| Analysis Comments: | HSQC dataset was collected using the custom 1.5 mm HTS probe |
| Software Version: | VNMRJ |
| Operator Name: | Chaevien Clendinen |
| Randomization Order: | NMR/Processed |
| Data Format: | Varian FID |
| Chromatography ID: | CH000112 |
| Num Factors: | 3 |
| Analysis ID: | AN000161 |
| Laboratory Name: | Arthur Edison |
| Analysis Type: | NMR |
| Analysis Comments: | HSQC-TOCSY dataset was collected using the custom 1.5 mm HTS prob |
| Software Version: | VNMRJ |
| Operator Name: | Chaevien Clendinen |
| Randomization Order: | NMR/Processed |
| Data Format: | Varian FID |
| Chromatography ID: | CH000112 |
| Num Factors: | 3 |
| Analysis ID: | AN000162 |
| Laboratory Name: | Arthur Edison |
| Analysis Type: | NMR |
| Analysis Comments: | Carbon 1D dataset was all collected using the custom 1.5 mm HTS prob |
| Software Version: | VNMRJ |
| Operator Name: | Chaevien Clendinen |
| Randomization Order: | NMR/Processed |
| Data Format: | Varian FID |
| Chromatography ID: | CH000112 |
| Num Factors: | 3 |
NMR:
| NMR ID: | NM000029 |
| Analysis ID: | AN000160 |
| Instrument Name: | Agilent VNMRS-600 |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 2D 1H-13C HSQC |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | custome 1.5 mm HTS probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 1.5 mm |
| Pulse Width: | 90d |
| Power Level: | 37 |
| Offset Frequency: | 4.77 |
| Temperature: | 23d |
| Number Of Scans: | 32 |
| Dummy Scans: | 16 |
| Acquisition Time: | 0.14 |
| Relaxation Delay: | 1.5 sec |
| Spectral Width: | 12 ppm ; 205.5 ppm |
| Num Data Points Acquired: | 2048 |
| Real Data Points: | 1024 |
| Line Broadening: | 2 |
| Zero Filling: | 2x |
| Apodization: | EM |
| Baseline Correction Method: | POLY |
| NMR ID: | NM000030 |
| Analysis ID: | AN000161 |
| Instrument Name: | Agilent VNMRS-600 |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 2D 1H-13C HSQC-TOCSY |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | custome 1.5 mm HTS probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 1.5 mm |
| Pulse Width: | 90d |
| Power Level: | 37 |
| Offset Frequency: | 4.77 |
| Temperature: | 23d |
| Number Of Scans: | 96 |
| Dummy Scans: | 16 |
| Acquisition Time: | 0.14 |
| Relaxation Delay: | 1.5 sec |
| Spectral Width: | 13 ppm ; 205.5 ppm/14 ppm ; 205.5 ppm |
| Num Data Points Acquired: | 2048 |
| Real Data Points: | 1024 |
| Line Broadening: | 2 |
| Zero Filling: | 2x |
| Apodization: | EM |
| Baseline Correction Method: | POLY |
| NMR ID: | NM000031 |
| Analysis ID: | AN000162 |
| Instrument Name: | Agilent VNMRS-600 |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 13C |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | custome 1.5 mm HTS probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 1.5 mm |
| Pulse Width: | 60d |
| Power Level: | 57 |
| Temperature: | 23d |
| Number Of Scans: | 512 |
| Dummy Scans: | 16 |
| Relaxation Delay: | 2 sec |
| Spectral Width: | 212 ppm |
| Num Data Points Acquired: | 96154 |
| Real Data Points: | 48077 |
| Line Broadening: | 5 |
| Zero Filling: | 2x |
| Apodization: | EM |
| Baseline Correction Method: | POLY |
