Summary of Study ST000148
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000127. The data can be accessed directly via it's Project DOI: 10.21228/M8959T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000148 |
| Study Title | A549 13C-labeled Cell Study |
| Study Type | tracer |
| Study Summary | [U-13C]-Glu SIRM study of A549 cells with QDA treatment |
| Institute | University of Kentucky |
| Department | CESB |
| Last Name | Fan |
| First Name | Teresa |
| Address | Rm 516 Biopharm Complex, 789 S. Limestone St.,Univ. of Kentucky, Lexington, KY 40536 |
| twmfan@gmail.com | |
| Submit Date | 2015-03-24 |
| Raw Data Available | No |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-03-24 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000127 |
| Project DOI: | doi: 10.21228/M8959T |
| Project Title: | Evaluation of QDA chemoselection |
| Project Summary: | Demonstrate the effectiveness of QDA/13CD3-QDA chemoselection experimental protocol on stable isotope tracer based metabolomic investigation of cell metabolism |
| Institute: | University of Kentucky |
| Department: | CESB |
| Last Name: | Fan |
| First Name: | Teresa |
| Address: | Rm 516 Biopharm Complex, 789 S. Limestone St.,Univ. of Kentucky, Lexington, KY 40536 |
| Email: | twmfan@gmail.com |
Subject:
| Subject ID: | SU000167 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | A549 |
| Species Group: | Mammals |
Factors:
Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA008085 | A549_Ctl_3_13C6Glc_plate | 13C-Glc |
| SA008086 | A549_Ctl_1_13C6Glc_plate | 13C-Glc |
| SA008087 | A549_Ctl_2_13C6Glc_plate | 13C-Glc |
| SA008088 | A549_Ctl_1_unL_plate | unlabeled |
| SA008089 | A549_Ctl_2_unL_plate | unlabeled |
| Showing results 1 to 5 of 5 |
Collection:
| Collection ID: | CO000153 |
| Collection Summary: | - |
| Collection Protocol Filename: | WS_Fan_Quench_Cell_Tissue.pdf |
| Collection Protocol Comments: | Collection and quench of cells in tissue culture |
| Sample Type: | Epithelial cells |
Treatment:
| Treatment ID: | TR000172 |
| Treatment Summary: | unlabeled | 13C-Glc |
| Treatment Protocol Filename: | WS_Fan_Cell_Tracer_Ex.pdf |
| Treatment Protocol Comments: | cell culture in unlabeled media for 24h with QDA derivatives | cell culture in 13C-Glc-labeled media for 24h with QDA derivatives |
Sample Preparation:
| Sampleprep ID: | SP000167 |
| Sampleprep Summary: | polar extraction | protein extraction | lipid extraction |
| Sampleprep Protocol Filename: | WS_Fan_Extract_Polar_Lipid_Prot.pdf_ _WS_Fan_Prot_Quant.pdf_ _WS_Fan_Extract_Polar_Lipid_Prot.pdf |
| Sampleprep Protocol Comments: | Polar extraction from cells | Protein extraction and quantification | Lipid extraction from cells |
Combined analysis:
| Analysis ID | AN000235 |
|---|---|
| Analysis type | MS |
| Chromatography type | |
| Chromatography system | |
| Column | |
| MS Type | ESI |
| MS instrument type | FT-ICR-MS |
| MS instrument name | Bruker SolariX FT-ICR-MS |
| Ion Mode | POSITIVE |
| Units | normalized corrected Peak area |
Chromatography:
| Chromatography ID: | CH000164 |
MS:
| MS ID: | MS000186 |
| Analysis ID: | AN000235 |
| Instrument Name: | Bruker SolariX FT-ICR-MS |
| Instrument Type: | FT-ICR-MS |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
