Summary of Study ST000163

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000141. The data can be accessed directly via it's Project DOI: 10.21228/M8R59V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000163
Study TitleMetabolomics of 50 healthy humans and common marmosets (MetabNet)
Study TypeHealthy subjects
Institute
Emory University
DepartmentDepartment of Medicine
LaboratoryClinical Biomarkers Laboratory
Last NameUppal
First NameKaran
AddressWhitehead Biomedical Research Building, Rm 225, 621 Michael Street, Atlanta, GA 30322
Emailkuppal2@emory.edu
Submit Date2015-04-27
Total Subjects100
Raw Data AvailableYes
Raw Data File Type(s)cdf, raw(Thermo)
Uploaded File Size59 G
Analysis Type DetailLC-MS
Release Date2015-04-28
Release Version1
Karan Uppal Karan Uppal
https://dx.doi.org/10.21228/M8R59V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000141
Project DOI:doi: 10.21228/M8R59V
Project Title:Metabolomics of 50 healthy humans and common marmosets
Institute:Emory University
Department:Department of Medicine
Laboratory:Clinical Biomarkers Laboratory
Last Name:Jones
First Name:Dean
Address:Whitehead Biomedical Research Building, Rm 225, 621 Michael Street, Atlanta, GA 30322
Email:kuppal2@emory.edu

Subject:

Subject ID:SU000182
Subject Type:Human/animal
Subject Species:Homo sapiens;Callithrix jacchus
Taxonomy ID:9606;9483
Species Group:Mammal

Factors:

Subject type: Human/animal; Subject species: Homo sapiens;Callithrix jacchus (Factor headings shown in green)

mb_sample_id local_sample_id Species
SA008623VT_130306_088Human
SA008624VT_130306_090Human
SA008625VT_130306_092Human
SA008626VT_130306_086Human
SA008627VT_130306_084Human
SA008628VT_130306_078Human
SA008629VT_130306_080Human
SA008630VT_130306_082Human
SA008631VT_130306_094Human
SA008632VT_130306_096Human
SA008633VT_130306_106Human
SA008634VT_130306_108Human
SA008635VT_130306_110Human
SA008636VT_130306_104Human
SA008637VT_130306_102Human
SA008638VT_130306_098Human
SA008639VT_130306_100Human
SA008640VT_130306_076Human
SA008641VT_130306_074Human
SA008642VT_130306_050Human
SA008643VT_130306_052Human
SA008644VT_130306_054Human
SA008645VT_130306_048Human
SA008646VT_130306_046Human
SA008647VT_130306_040Human
SA008648VT_130306_042Human
SA008649VT_130306_044Human
SA008650VT_130306_056Human
SA008651VT_130306_058Human
SA008652VT_130306_068Human
SA008653VT_130306_070Human
SA008654VT_130306_072Human
SA008655VT_130306_066Human
SA008656VT_130306_064Human
SA008657VT_130306_060Human
SA008658VT_130306_062Human
SA008659VT_130306_112Human
SA008660VT_130306_114Human
SA008661VT_130307_044Human
SA008662VT_130307_046Human
SA008663VT_130307_048Human
SA008664VT_130307_042Human
SA008665VT_130307_040Human
SA008666VT_130307_034Human
SA008667VT_130307_036Human
SA008668VT_130307_038Human
SA008669VT_130307_050Human
SA008670VT_130307_052Human
SA008671VT_130307_062Human
SA008672VT_130307_064Human
SA008673VT_130304_014Human
SA008674VT_130307_060Human
SA008675VT_130307_058Human
SA008676VT_130307_054Human
SA008677VT_130307_056Human
SA008678VT_130307_032Human
SA008679VT_130307_030Human
SA008680VT_130306_126Human
SA008681VT_130307_008Human
SA008682VT_130307_010Human
SA008683VT_130306_124Human
SA008684VT_130306_122Human
SA008685VT_130306_116Human
SA008686VT_130306_118Human
SA008687VT_130306_120Human
SA008688VT_130307_012Human
SA008689VT_130307_014Human
SA008690VT_130307_024Human
SA008691VT_130307_026Human
SA008692VT_130307_028Human
SA008693VT_130307_022Human
SA008694VT_130307_020Human
SA008695VT_130307_016Human
SA008696VT_130307_018Human
SA008697VT_130306_038Human
SA008698VT_130307_066Human
SA008699VT_130304_064Human
SA008700VT_130304_066Human
SA008701VT_130304_068Human
SA008702VT_130304_062Human
SA008703VT_130304_060Human
SA008704VT_130304_054Human
SA008705VT_130304_056Human
SA008706VT_130304_058Human
SA008707VT_130304_070Human
SA008708VT_130304_072Human
SA008709VT_130304_082Human
SA008710VT_130304_084Human
SA008711VT_130304_086Human
SA008712VT_130304_080Human
SA008713VT_130304_078Human
SA008714VT_130304_074Human
SA008715VT_130304_076Human
SA008716VT_130306_036Human
SA008717VT_130304_050Human
SA008718VT_130304_026Human
SA008719VT_130304_028Human
SA008720VT_130304_030Human
SA008721VT_130304_024Human
SA008722VT_130304_022Human
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Collection:

Collection ID:CO000168
Collection Summary:-

Treatment:

Treatment ID:TR000188
Treatment Summary:No treatment

Sample Preparation:

Sampleprep ID:SP000182
Sampleprep Summary:aliquots of 65ul sample were mixed with 130ul acetonitrile and 3.25ul internal standard. Vortex for 1 minute on high and leave on ice for 30 minutes. Centrifuge for 10 minutes at 13.2rpm. 130ul of supernatant was removed and placed into autosampler vials for the mass spectrometer.
Processing Method:precipitation of protein, centrifuge, and remove supernatant
Processing Storage Conditions:on ice
Extraction Method:1:2 sample:acetonitrile
Extract Storage:supernatant pipette into autosampler vials for mass spectrometer
Sample Spiking:internal standards: [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, 2'-deoxyguanosine-13C10,15N5-5'-monophosphate

Combined analysis:

Analysis ID AN000255
Analysis type MS
Chromatography type Anion exchange/Reversed phase
Chromatography system Thermo Accela CTC
Column Higgins C18 (100 x 2.1mm,5um); Hamilton Anion Exchange 100x2.1mm,7um
MS Type ESI
MS instrument type Single quadrupole
MS instrument name Thermo LTQ
Ion Mode POSITIVE
Units Intensity

Chromatography:

Chromatography ID:CH000180
Chromatography Summary:untargeted dual chromatography (C18 and Anion Exchange column)
Methods ID:Methods_LTQVelosOrbi
Methods Filename:Methods_LTQVelosOrbi
Instrument Name:Thermo Accela CTC
Column Name:Higgins C18 (100 x 2.1mm,5um); Hamilton Anion Exchange 100x2.1mm,7um
Column Pressure:200 Bar (max)
Column Temperature:30
Flow Gradient:C18: A% = 5% for 10 minutes, 0-2mins = 60% B, 2-6mins = gradient to 95% B, 6-10mins = 95% B; AE: 1-10mins = 50%B, 0-2mins = 5%A, 2-7mins = gradient to 50%A, 7-10mins = 50%A
Flow Rate:0.35 mL/min
Injection Temperature:4C
Sample Injection:10ul
Sampling Cone:ESI
Solvent A:100% water; 0.2% formic acid
Solvent B:100% acetonitrile
Analytical Time:LC grade water
Capillary Voltage:10 minutes
Migration Time:100uA (source current)
Sheath Liquid:4.5kV (source voltage)
Weak Wash Solvent Name:water with 10% methanol
Sample Syringe Size:20ul
Chromatography Type:Anion exchange/Reversed phase

MS:

MS ID:MS000205
Analysis ID:AN000255
Instrument Name:Thermo LTQ
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Capillary Temperature:4.45
Collision Energy:N2
Fragmentation Method:50psi
Ion Spray Voltage:electrospray ionization
Ionization Potential:5ppm
Reagent Gas:48
Atom Gun Current:1000000
Cdl Temperature:.raw
Sample Dripping:85-2000
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