Summary of Study ST000232
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000182. The data can be accessed directly via it's Project DOI: 10.21228/M8M888 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000232 |
| Study Title | Untargeted metabolomic analysis of the small intestinal content of malnourished mice |
| Study Type | Metabolomics |
| Study Summary | A total of 8 samples from 6 week old, female C57BL/6 mice, treated for 3 weeks with a malnourished diet or a control-fed isocaloric diet. Samples were taken from the small intestinal fecal content at the terminus of the ileum. |
| Institute | University of Victoria |
| Department | The Uvic Proteomics and Metabolomics Innovation Centre |
| Last Name | Borchers |
| First Name | Christoph |
| Address | #3101-4464 Markham St Victoria, BC Canada, V8Z 7X8 |
| christoph@proteincentre.com | |
| Submit Date | 2015-06-01 |
| Num Groups | 2 |
| Total Subjects | 8 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Uploaded File Size | 1.1 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-08-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000182 |
| Project DOI: | doi: 10.21228/M8M888 |
| Project Title: | Metabolomic analysis of the small intestinal content of malnourished mice |
| Institute: | University of British Columbia |
| Department: | Microbiology and Immunology |
| Laboratory: | Dr. B. Brett Finlay |
| Last Name: | Finlay |
| First Name: | Brett |
| Address: | 2185 East Mall, Vancouver, BC, Canada V6T 1Z4 |
| Email: | bfinlay@msl.ubc.ca |
| Funding Source: | Canadian Institutes for Health Research |
Subject:
| Subject ID: | SU000251 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 6 weeks at collection |
| Gender: | Female |
| Animal Animal Supplier: | Jackson Labs |
| Animal Housing: | Conventional |
| Animal Feed: | Low Protein, Low Fat Malnourished Diet|Isocaloric Control Diet |
| Subject Comments: | 2 subjects |
| Cell Primary Immortalized: | Malnourished|Control |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diet |
|---|---|---|
| SA010877 | CON2 | control |
| SA010878 | CON3 | control |
| SA010879 | CON4 | control |
| SA010880 | CON1 | control |
| SA010881 | MAL4 | malnourished |
| SA010882 | MAL2 | malnourished |
| SA010883 | MAL3 | malnourished |
| SA010884 | MAL1 | malnourished |
| Showing results 1 to 8 of 8 |
Collection:
| Collection ID: | CO000241 |
| Collection Summary: | - |
| Sample Type: | Small intestinal fecal contents |
| Collection Time: | 6 weeks of age |
| Storage Conditions: | -80 degrees |
| Tissue Cell Quantity Taken: | 15-40mg |
Treatment:
| Treatment ID: | TR000261 |
| Treatment Summary: | 4 mice were given a malnourished diet and 4 mice were untreated, fed a control diet |
Sample Preparation:
| Sampleprep ID: | SP000255 |
| Sampleprep Summary: | Metabolite Extraction. Mouse small intestine digesta samples (luminal contents) were collected, weighed and homogenized in water followed by addition of acetonitrile for metabolite extraction. After vortex mixing, 30 second sonication, and centrifugation, the clear supernatants were collected and dried under a gentle nitrogen gas flow. The dried metabolite residues were dissolved in 40% acetonitrile containing 0.01% formic acid, 4 ?L per mg of the raw material. After 15 sec vortex and 30 sec sonication, each sample was further diluted 1:2 with water. Twelve-?L aliquots were injected for untargeted metabolic fingerprinting by ultrahigh-performance liquid chromatography ? Fourier transform mass spectrometry (UPLC-FTMS). |
Combined analysis:
| Analysis ID | AN000347 | AN000348 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | ||
| Column | C18 | C18 |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Relative Abundance | Relative Abundance |
Chromatography:
| Chromatography ID: | CH000259 |
| Column Name: | C18 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000293 |
| Analysis ID: | AN000347 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000294 |
| Analysis ID: | AN000348 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
