Summary of Study ST000571
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000419. The data can be accessed directly via it's Project DOI: 10.21228/M8WK63 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000571 |
| Study Title | Metabolomics of uterine fluid of the cow |
| Study Type | time course |
| Study Summary | Day 0, 3, 5 and 7 after estrus |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | P. J. Hansen |
| Last Name | Moss |
| First Name | James |
| Address | 2250 SW Shealy Dr., Gainesville FL 32611 |
| dushka@ufl.edu | |
| Phone | (352) 339-2237 |
| Submit Date | 2017-01-13 |
| Num Groups | 4 |
| Total Subjects | 15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000419 |
| Project DOI: | doi: 10.21228/M8WK63 |
| Project Title: | Metabolomics of uterine fluid of the cow |
| Project Type: | Research |
| Project Summary: | The goal is to determine cyclic changes in the metabolome of uterine fluid |
| Institute: | University of Florida |
| Department: | IFAS |
| Last Name: | Hansen |
| First Name: | Peter |
| Address: | PO Box 110910 |
| Email: | Hansen@animal.ufl.edu |
| Phone: | 352-359-5753 |
| Funding Source: | USDA-NIFA |
Subject:
| Subject ID: | SU000593 |
| Subject Type: | Animal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Genotype Strain: | Holstein |
| Age Or Age Range: | Adult |
| Gender: | Female |
| Animal Animal Supplier: | University of Florida |
| Animal Housing: | Pasture |
| Animal Light Cycle: | Natural |
| Animal Feed: | Pasture |
| Animal Water: | Ad Lib |
| Animal Inclusion Criteria: | Cyclic |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Bos taurus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Days after ovulation |
|---|---|---|
| SA029942 | PJH_001 | D0 |
| SA029943 | PJH_004 | D0 |
| SA029944 | PJH_003 | D0 |
| SA029945 | PJH_002 | D0 |
| SA029946 | PJH_007 | D3 |
| SA029947 | PJH_008 | D3 |
| SA029948 | PJH_006 | D3 |
| SA029949 | PJH_005 | D3 |
| SA029950 | PJH_011 | D5 |
| SA029951 | PJH_010 | D5 |
| SA029952 | PJH_009 | D5 |
| SA029953 | PJH_015 | D7 |
| SA029954 | PJH_012 | D7 |
| SA029955 | PJH_013 | D7 |
| SA029956 | PJH_014 | D7 |
| Showing results 1 to 15 of 15 |
Collection:
| Collection ID: | CO000587 |
| Collection Summary: | Recover uterus at slaughter; flush uterine lumen with 30 ml DPBS |
| Sample Type: | Uterine fluid |
| Collection Method: | Flush |
| Volumeoramount Collected: | 30 ml |
| Storage Conditions: | -20 C |
Treatment:
| Treatment ID: | TR000607 |
| Treatment Summary: | Uterine flushings collected at Day 0, 3, 5 and 7 after estrus |
Sample Preparation:
| Sampleprep ID: | SP000600 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
| Analysis ID | AN000879 | AN000880 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000624 |
| Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
| Chromatography Comments: | Ensure that Column is an ACE Excel 2 C18-PFP with dimensions of 100 x 2.1mm, 2.0 μm with a Halo C18-PFP guard attached |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000780 |
| Analysis ID: | AN000879 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
| MS ID: | MS000781 |
| Analysis ID: | AN000880 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
