Summary of Study ST000668

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000471. The data can be accessed directly via it's Project DOI: 10.21228/M85P6M This work is supported by NIH grant, U2C- DK119886.

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Study IDST000668
Study TitleDiet manipulation on the lipidome
Study TypeMS analysis
Study SummarySubjects were subjected to different dietary interventions. Indivduals (A1 to A19) were either randomized to a isocaloric diet with either 40-45% saturated fat diet or 40-45% monounsaturated fat diet for 4 weeks. Plasma was sampled at baseline and after 4 weeks on the diet in the fasting state. Multiple clinical values (Weight, blood pressure, pulse,total cholesterol, HDL, LDL and triglycerides) and hyperinsulinemic euglycemic clamps were assessed. Alternatively (XS-3 to XS 43) were provided a diet that was 2000 calories above the estimated isocaloric state for 2 weeks. Multiple clinical values (Weight, blood pressure, pulse,total cholesterol, HDL, LDL and triglycerides) were determined along with body composition, insulin and glucose values.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups6
Total Subjects22
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M85P6M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000471
Project DOI:doi: 10.21228/M85P6M
Project Title:Dietary macronutrient intake prediction by metabolomics
Project Type:MS analysis
Project Summary:Effect of diet on metabolome
Institute:University of Michigan
Department:Internal Medicine
Laboratory:Burant Lab
Last Name:Burant
First Name:Chuck
Address:1500 E. Medical Center Drive Ann Arbor, MI 48109
Email:burantc@umich.edu
Phone:734 615-3481

Subject:

Subject ID:SU000691
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Diet intervention Gender
SA038452S00019202Baseline female
SA038453S00019200Baseline female
SA038454S00019196Baseline female
SA038455S00019182Baseline female
SA038456S00019198Baseline female
SA038457S00019208Baseline female
SA038458S00019224Baseline female
SA038459S00019216Baseline female
SA038460S00019214Baseline female
SA038461S00019194Baseline female
SA038462S00019206Baseline female
SA038463S00019204Baseline female
SA038464S00019188Baseline female
SA038465S00019192Baseline female
SA038466S00019186Baseline female
SA038467S00019184Baseline female
SA038468S00019190Baseline female
SA038469S00019212Baseline male
SA038470S00019220Baseline male
SA038471S00019222Baseline male
SA038472S00019218Baseline male
SA038473S00019210Baseline male
SA038474S00019225Overfeeding-2 weeks female
SA038475S00019217Overfeeding-2 weeks female
SA038476S00019215Overfeeding-2 weeks female
SA038477S00019221Overfeeding-2 weeks male
SA038478S00019223Overfeeding-2 weeks male
SA038479S00019213Overfeeding-2 weeks male
SA038480S00019211Overfeeding-2 weeks male
SA038481S00019219Overfeeding-2 weeks male
SA038482S00019191Saturated Fatty Acid female
SA038483S00019185Saturated Fatty Acid female
SA038484S00019187Saturated Fatty Acid female
SA038485S00019193Saturated Fatty Acid female
SA038486S00019183Saturated Fatty Acid female
SA038487S00019195Saturated Fatty Acid female
SA038488S00019189Saturated Fatty Acid female
SA038489S00019197Unsaturated Fatty Acid female
SA038490S00019207Unsaturated Fatty Acid female
SA038491S00019209Unsaturated Fatty Acid female
SA038492S00019203Unsaturated Fatty Acid female
SA038493S00019201Unsaturated Fatty Acid female
SA038494S00019205Unsaturated Fatty Acid female
SA038495S00019199Unsaturated Fatty Acid female
Showing results 1 to 44 of 44

Collection:

Collection ID:CO000685
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000705
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000698
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001022 AN001023
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000731
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS000917
Analysis ID:AN001022
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS000918
Analysis ID:AN001023
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
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