Summary of Study ST000932

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000187. The data can be accessed directly via it's Project DOI: 10.21228/M8388M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000932
Study TitleActivity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv0100
Study Typetimecourse
Study SummaryThese experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze.
Institute
Weill Cornell Medicine
Last NameRhee
First NameKyu
AddressDepartment of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY
Emailkyr9001@med.cornell.edu
Phone979-229-1494
Submit Date2018-03-01
Analysis Type DetailLC-MS
Release Date2018-04-10
Release Version1
Kyu Rhee Kyu Rhee
https://dx.doi.org/10.21228/M8388M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000187
Project DOI:doi: 10.21228/M8388M
Project Title:FLUTE: Functionalizing Lists of Unknown TB Entities
Project Type:NIH U19
Project Summary:functional genomics of proteins of unknown function in M. tuberculosis
Institute:Weill Cornell Medicine
Department:Microbiology and Immunology
Last Name:Rhee
First Name:Kyu
Address:#N/A
Email:kyr9001@med.cornell.edu

Subject:

Subject ID:SU000971
Subject Type:Bacteria
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332

Factors:

Subject type: Bacteria; Subject species: Mycobacterium tuberculosis H37Rv (Factor headings shown in green)

mb_sample_id local_sample_id timepoint (min) protein prep
SA0555540100_B_1515 Active
SA0555550100_A_1515 Active
SA0555560100_C_1515 Active
SA055557Inactivated-0100_C_1515 Inactive
SA055558Inactivated-0100_B_1515 Inactive
SA055559Inactivated-0100_A_1515 Inactive
SA0555600100_C_3030 Active
SA0555610100_B_3030 Active
SA0555620100_A_3030 Active
SA055563Inactivated-0100_C_3030 Inactive
SA055564Inactivated-0100_A_3030 Inactive
SA055565Inactivated-0100_B_3030 Inactive
SA0555480100_A_0- Active
SA0555490100_C_0- Active
SA0555500100_B_0- Active
SA055551Inactivated-0100_B_0- Inactive
SA055552Inactivated-0100_C_0- Inactive
SA055553Inactivated-0100_A_0- Inactive
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000965
Collection Summary:Rv0100 from M. tuberculosis H37Rv was expressed in E. coli cells and purified.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000985
Treatment Summary:Protein was incubated with whole-cell lysate in either Active form in Inactive (heat-inactivated).

Sample Preparation:

Sampleprep ID:SP000978
Sampleprep Summary:Protein was incubated with whole-cell lysate in either Active form or Inactive (heat-inactivated).

Combined analysis:

Analysis ID AN001527 AN001528
Analysis type MS MS
Chromatography type Normal phase Normal phase
Chromatography system Agilent 1290 Agilent 1290
Column Microsolv Diamond hydride (150mm,4um) Microsolv Diamond hydride (150mm,4um)
MS Type ESI ESI
MS instrument type TOF TOF
MS instrument name Agilent 6220 TOF Agilent 6220 TOF
Ion Mode POSITIVE NEGATIVE
Units peak counts peak counts

Chromatography:

Chromatography ID:CH001076
Chromatography Summary:Aqueous normal phase diamond hydride
Instrument Name:Agilent 1290
Column Name:Microsolv Diamond hydride (150mm,4um)
Chromatography Type:Normal phase

MS:

MS ID:MS001407
Analysis ID:AN001527
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001408
Analysis ID:AN001528
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:NEGATIVE
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