Summary of Study ST000935
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000187. The data can be accessed directly via it's Project DOI: 10.21228/M8388M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000935 |
| Study Title | Activity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv2553c |
| Study Type | timecourse |
| Study Summary | These experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze. |
| Institute | Weill Cornell Medicine |
| Last Name | Rhee |
| First Name | Kyu |
| Address | Department of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY |
| kyr9001@med.cornell.edu | |
| Phone | 979-229-1494 |
| Submit Date | 2018-03-03 |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-04-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000187 |
| Project DOI: | doi: 10.21228/M8388M |
| Project Title: | FLUTE: Functionalizing Lists of Unknown TB Entities |
| Project Type: | NIH U19 |
| Project Summary: | functional genomics of proteins of unknown function in M. tuberculosis |
| Institute: | Weill Cornell Medicine |
| Department: | Microbiology and Immunology |
| Last Name: | Rhee |
| First Name: | Kyu |
| Address: | #N/A |
| Email: | kyr9001@med.cornell.edu |
Subject:
| Subject ID: | SU000974 |
| Subject Type: | Bacteria |
| Subject Species: | Mycobacterium tuberculosis H37Rv |
| Taxonomy ID: | 83332 |
Factors:
Subject type: Bacteria; Subject species: Mycobacterium tuberculosis H37Rv (Factor headings shown in green)
| mb_sample_id | local_sample_id | timepoint (min) | protein prep |
|---|---|---|---|
| SA055654 | 2553_B_15 | 15 | Active |
| SA055655 | 2553_A_15 | 15 | Active |
| SA055656 | 2553_C_15 | 15 | Active |
| SA055657 | Inactivated-2553_C_15 | 15 | Inactive |
| SA055658 | Inactivated-2553_B_15 | 15 | Inactive |
| SA055659 | Inactivated-2553_A_15 | 15 | Inactive |
| SA055660 | 2553_C_30 | 30 | Active |
| SA055661 | 2553_B_30 | 30 | Active |
| SA055662 | 2553_A_30 | 30 | Active |
| SA055663 | Inactivated-2553_C_30 | 30 | Inactive |
| SA055664 | Inactivated-2553_A_30 | 30 | Inactive |
| SA055665 | Inactivated-2553_B_30 | 30 | Inactive |
| SA055648 | 2553_A_0 | - | Active |
| SA055649 | 2553_C_0 | - | Active |
| SA055650 | 2553_B_0 | - | Active |
| SA055651 | Inactivated-2553_B_0 | - | Inactive |
| SA055652 | Inactivated-2553_C_0 | - | Inactive |
| SA055653 | Inactivated-2553_A_0 | - | Inactive |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO000968 |
| Collection Summary: | Rv2553c from M. tuberculosis H37Rv was expressed in E. coli cells and purified. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR000988 |
| Treatment Summary: | Protein was incubated with whole-cell lysate in either Active form in Inactive (heat-inactivated). Protein was incubated with whole-cell lysate in either Active form in Inactive (heat-inactivated). |
Sample Preparation:
| Sampleprep ID: | SP000981 |
| Sampleprep Summary: | Protein was incubated with whole-cell lysate in either Active form or Inactive (heat-inactivated). Protein was incubated with whole-cell lysate in either Active form or Inactive(heat-inactivated). |
Combined analysis:
| Analysis ID | AN001532 | AN001533 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Normal phase | Normal phase |
| Chromatography system | Agilent 1290 | Agilent 1290 |
| Column | Microsolv Diamond hydride (150mm,4um) | Microsolv Diamond hydride (150mm,4um) |
| MS Type | ESI | ESI |
| MS instrument type | TOF | TOF |
| MS instrument name | Agilent 6220 TOF | Agilent 6220 TOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak counts | peak counts |
Chromatography:
| Chromatography ID: | CH001079 |
| Chromatography Summary: | Aqueous normal phase diamond hydride |
| Instrument Name: | Agilent 1290 |
| Column Name: | Microsolv Diamond hydride (150mm,4um) |
| Chromatography Type: | Normal phase |
MS:
| MS ID: | MS001412 |
| Analysis ID: | AN001532 |
| Instrument Name: | Agilent 6220 TOF |
| Instrument Type: | TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS001413 |
| Analysis ID: | AN001533 |
| Instrument Name: | Agilent 6220 TOF |
| Instrument Type: | TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
