Summary of Study ST001050

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000703. The data can be accessed directly via it's Project DOI: 10.21228/M8T10P This work is supported by NIH grant, U2C- DK119886.

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Study IDST001050
Study TitleP4HA1 knockdown in the breast cell line MDA231 Gln metabolism (part II)
Study Typeisotope tracer
Study SummaryDetermine the effect on glutamine metabolism of knocking down P4HA1 in the MDA231 cell line.
Institute
University of Kentucky
DepartmentMarkey Cancer Center
Last NameXiong
First NameGaofeng
AddressBBSRC361, 741 South Limestone, Lexington, KY 40536, USA
Emailgaofeng.xiong@uky.edu
Phone000-000-0000
Submit Date2018-09-12
Study CommentsFor additional comments about the study, like listing a publication.
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC-MS
Release Date2019-03-06
Release Version1
Gaofeng Xiong Gaofeng Xiong
https://dx.doi.org/10.21228/M8T10P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000703
Project DOI:doi: 10.21228/M8T10P
Project Title:P4HA1 knockdown in the breast cell line MDA231
Project Summary:Determine the effect of knocking down P4HA1 in the MDA231 cell line (including alterations to metabolic pathways).
Institute:University of Kentucky
Department:Markey Cancer Center
Last Name:Xu
First Name:Ren
Address:BBSRC361, 741 South Limestone, Lexington, KY 40536, USA
Email:ren.xu2010@uky.edu
Phone:000-000-0000

Subject:

Subject ID:SU001199
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id protocol.id
SA0782671_MDA231_12C14NGln_shCTL_rep1-cells-polar_GCMS_A12C14NGln_shCTL
SA0782685_MDA231_12C14NGln_shP4HA1_rep1-cells-polar_GCMS_A12C14NGln_shP4HA1
SA0782692_MDA231_13C15NGln_shCTL_rep1-cells-polar_GCMS_A13C15NGln_shCTL
SA0782704_MDA231_13C15NGln_shCTL_rep3-cells-polar_GCMS_A13C15NGln_shCTL
SA0782713_MDA231_13C15NGln_shCTL_rep2-cells-polar_GCMS_A13C15NGln_shCTL
SA0782728_MDA231_13C15NGln_shP4HA1_rep3-cells-polar_GCMS_A13C15NGln_shP4HA1
SA0782736_MDA231_13C15NGln_shP4HA1_rep1-cells-polar_GCMS_A13C15NGln_shP4HA1
SA0782747_MDA231_13C15NGln_shP4HA1_rep2-cells-polar_GCMS_A13C15NGln_shP4HA1
Showing results 1 to 8 of 8

Collection:

Collection ID:CO001193
Collection Summary:Collection and quench of cells in culture
Collection Protocol ID:cell_quench_collection
Collection Protocol Filename:3A_Cells_Quench_Cell_Fan_20170412.pdf
Sample Type:cultured cells

Treatment:

Treatment ID:TR001214
Treatment Summary:Cells grown in unlabeled glutamine and infected with empty vector. Cells grown in unlabeled glutamine and infected with shP4HA1 lentivirus. Cells grown in labeled glutamine and infected with empty vector. Cells grown in labeled glutamine and infected with shP4HA1 lentivirus.
Treatment Protocol ID:12C14NGln_shCTL 12C14NGln_shP4HA1 13C15NGln_shCTL 13C15NGln_shP4HA1

Sample Preparation:

Sampleprep ID:SP001207
Sampleprep Summary:Polar extraction from cells. Protein extraction and quantification.
Sampleprep Protocol ID:polar_extraction protein_extraction
Sampleprep Protocol Filename:4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf
4D_17Jun4_Fan_Prot_Quant.pdf

Combined analysis:

Analysis ID AN001865
Analysis type MS
Chromatography type GC
Chromatography system GC-EI-MS, Thermo ITQ
Column SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
MS Type EI
MS instrument type GC-ITQ
MS instrument name Thermo ITQ
Ion Mode POSITIVE
Units normalized peak area

Chromatography:

Chromatography ID:CH001350
Chromatography Summary:Targeted GC
Instrument Name:GC-EI-MS, Thermo ITQ
Column Name:SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
Chromatography Type:GC

MS:

MS ID:MS001725
Analysis ID:AN001865
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
MS Comments:GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide).
Ion Mode:POSITIVE
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