Summary of Study ST001084
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000721. The data can be accessed directly via it's Project DOI: 10.21228/M8GM4Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001084 |
| Study Title | Multi-platform metabolomics data from Ndufs4-/- skeletal muscles (part -III) |
| Study Type | Untargeted GC-TOF-MS of Ndufs4-/- quadriceps muscles |
| Study Summary | Untargeted GC-TOF-MS analysis of Ndufs4-/- and WT mouse white quadriceps muscles. |
| Institute | North-West University |
| Last Name | Louw |
| First Name | Roan |
| Address | Hofman Street |
| Roan.Louw@nwu.ac.za | |
| Phone | +27 18 299 4074 |
| Submit Date | 2018-10-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | peg |
| Analysis Type Detail | GC-MS |
| Release Date | 2019-01-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000721 |
| Project DOI: | doi: 10.21228/M8GM4Z |
| Project Title: | Multi-platform metabolomics data from Ndufs4-/- skeletal muscles |
| Project Type: | Multi-platform metabolomics study |
| Project Summary: | Multi-platform metabolomics analysis of white quadriceps and soleus skeletal muscles from the Ndufs4 knockout (Ndufs4-/-) mouse model of human Leigh syndrome. |
| Institute: | North-West University |
| Last Name: | Louw |
| First Name: | Roan |
| Address: | Hofman Street |
| Email: | Roan.Louw@nwu.ac.za |
| Phone: | +27 18 299 4074 |
Subject:
| Subject ID: | SU001128 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
| Age Or Age Range: | 45-50 days |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
| Animal Light Cycle: | 12:12 h |
| Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
| Animal Water: | ad libitum |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA073349 | MQ_14 | Ndufs4-/- |
| SA073350 | MQ_17 | Ndufs4-/- |
| SA073351 | MQ_19 | Ndufs4-/- |
| SA073352 | MQ_12 | Ndufs4-/- |
| SA073353 | MQ_8 | Ndufs4-/- |
| SA073354 | MQ_4 | Ndufs4-/- |
| SA073355 | MQ_5 | Ndufs4-/- |
| SA073356 | MQ_21 | Ndufs4-/- |
| SA073357 | MQ_10 | Ndufs4-/- |
| SA073358 | MQ_24 | Ndufs4-/- |
| SA073359 | MQ_37 | Ndufs4-/- |
| SA073360 | MQ_38 | Ndufs4-/- |
| SA073361 | MQ_40 | Ndufs4-/- |
| SA073362 | MQ_35 | Ndufs4-/- |
| SA073363 | MQ_34 | Ndufs4-/- |
| SA073364 | MQ_2 | Ndufs4-/- |
| SA073365 | MQ_26 | Ndufs4-/- |
| SA073366 | MQ_29 | Ndufs4-/- |
| SA073367 | MQ_23 | Ndufs4-/- |
| SA073368 | MQ_39 | WT |
| SA073369 | MQ_13 | WT |
| SA073370 | MQ_15 | WT |
| SA073371 | MQ_16 | WT |
| SA073372 | MQ_18 | WT |
| SA073373 | MQ_11 | WT |
| SA073374 | MQ_9 | WT |
| SA073375 | MQ_3 | WT |
| SA073376 | MQ_6 | WT |
| SA073377 | MQ_7 | WT |
| SA073378 | MQ_20 | WT |
| SA073379 | MQ_22 | WT |
| SA073380 | MQ_32 | WT |
| SA073381 | MQ_33 | WT |
| SA073382 | MQ_36 | WT |
| SA073383 | MQ_31 | WT |
| SA073384 | MQ_30 | WT |
| SA073385 | MQ_25 | WT |
| SA073386 | MQ_27 | WT |
| SA073387 | MQ_28 | WT |
| SA073388 | MQ_1 | WT |
| Showing results 1 to 40 of 40 |
Collection:
| Collection ID: | CO001122 |
| Collection Summary: | Mice were euthanized between postnatal day (P) 45-50 via cervical dislocation at the same time of day (8:00-9:00 AM) after overnight (12-h) fasting. From both hind limbs, white muscle portions of quadriceps femoris, as well as soleus muscles were collected. Tissues were snap-frozen in liquid nitrogen (within 15 min post-mortem) and stored at -80°C until used. |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR001142 |
| Treatment Summary: | The animals did not receive any treatment. |
Sample Preparation:
| Sampleprep ID: | SP001135 |
| Sampleprep Summary: | Muscle tissues were finely minced and homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a modified monophasic Bligh–Dyer extraction method with a solvent ratio of 3:1:1 (methanol:water:chloroform). |
| Sampleprep Protocol Filename: | GC_quadriceps_sample_prep_protocol.pdf |
Combined analysis:
| Analysis ID | AN001769 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus HT TOF |
| Ion Mode | POSITIVE |
| Units | Normalized peak area |
Chromatography:
| Chromatography ID: | CH001249 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001635 |
| Analysis ID: | AN001769 |
| Instrument Name: | Leco Pegasus HT TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
