Summary of Study ST001379

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000916. The data can be accessed directly via it's Project DOI: 10.21228/M8912S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001379
Study Title2,3,7,8 -Tetrachlorodibenzo-p-dioxin Mediated Effects on Hepatic Coenzyme A (CoA) and Acetyl-CoA Levels (part-II)
Study TypeDioxin
Study SummaryExperiment to study TCDD-elicited effects on hepatic Coenzyme A (CoA) and Acetyl-CoA levels in liver of male mice treated with sesame oil vehicle or 0.01-30 ug/kg TCDD every 4 days for 28 days.
Institute
Michigan State University
DepartmentBiochemistry and Molecular Biology
LaboratoryZacharewski Lab
Last NameFling
First NameRussell
Address1129 Farm Ln, East Lansing, MI, USA
Emailflingrus@msu.edu
Phone(517) 884-2054
Submit Date2020-04-01
Num Groups9
Total Subjects80
Num Males29
Study CommentsFax: (517) 353-9334; ORCID: 0000-0002-6822-4962; ROLE: Graduate Student; Pubmed ID: -;Publication DOI: -;
Publications-
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2021-08-02
Release Version1
Russell Fling Russell Fling
https://dx.doi.org/10.21228/M8912S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000916
Project DOI:doi: 10.21228/M8912S
Project Title:TCDD-elicited effects on metabolite levels in liver of male mice treated with sesame oil
Project Type:Untargetd Metabolomics
Project Summary:Experiment to study TCDD-elicited effects on metabolite levels in liver of male mice treated with sesame oil vehicle or 0.01-30 ug/kg TCDD every 4 days for 28 days.
Institute:Michigan State University
Department:Biochemistry and Molecular Biology
Last Name:Fling
First Name:Russell
Address:1129 Farm Ln, East Lansing, MI,USA
Email:flingrus@msu.edu
Phone:(517) 884-2054
Publications:-

Subject:

Subject ID:SU001453
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6
Age Or Age Range:postnatal day 56
Gender:Male
Animal Housing:Cage Type: Innovive, Catalog#M-BTM, San Diego, CA; Bedding Type: cellulose fiber chips;
Animal Light Cycle:12:12
Animal Feed:22/5 Rodent Diet
Animal Water:Aquavive, Catalog#M-WB-300A, INNOVIVE, San Diego, CA

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id TCDD treatment(ug/kg)
SA100866mouse_330.3
SA100867mouse_350.3
SA100868mouse_380.3
SA100869mouse_340.3
SA100870mouse_370.3
SA100871mouse_431
SA100872mouse_461
SA100873mouse_441
SA100874mouse_421
SA100875mouse_481
SA100881mouse_6110
SA100882mouse_6410
SA100883mouse_6310
SA100884mouse_6010
SA100876mouse_493
SA100877mouse_593
SA100878mouse_533
SA100879mouse_563
SA100880mouse_503
SA100885mouse_6730
SA100886mouse_6830
SA100887mouse_6930
SA100888mouse_6630
SA100889mouse_6530
SA100890mouse_2vehicle
SA100891mouse_1vehicle
SA100892mouse_6vehicle
SA100893mouse_7vehicle
SA100894mouse_8vehicle
Showing results 1 to 29 of 29

Collection:

Collection ID:CO001448
Collection Summary:Mice were taken down with CO2. Livers were excised and immediately frozen in liquid nitrogen.
Collection Protocol Comments:Time-point: 28; Time-point Unit: DAYS;
Sample Type:Liver

Treatment:

Treatment ID:TR001468
Treatment Summary:oral gavage every 4 days for 28 days. 7 total exposures with sesame oil vehicle or 0.3-30 ug/kg TCDD.
Treatment Protocol Comments:Administration Interval: 4; Administration Interval Unit: DAYS; Dose Unit: ug/kg; Number of Administrations: 7; Chemical Soucre: AccuStandard; Chemical Catalog Number: S-70982; Chemical Purity: -; Vehicle Substance: CHEBI 28119
Treatment Compound:2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
Treatment Route:GAVAGE
Treatment Dose:0.3,1,3,10,30
Treatment Dosevolume:0.1 ml
Treatment Vehicle:Sesame oil
Animal Fasting:2 hrs before end-point
Animal Endp Euthanasia:CO2

Sample Preparation:

Sampleprep ID:SP001461
Sampleprep Summary:Frozen liver tissue was homegenized in 4.6 ml h20:methanol (5:3) mixture and homegenized, 2.9 ml chloroform was added,vortexed and spun down at 5,000G. Top layer was removed and blown down with nitrogen and reconstituted in 400 l 10mM tetrabutylammonium (TBA)+10mM acetate (pH 5). A further 1:16 dilution was performed for analysis of acetyl-CoA. Coenzyme A (CoA) was measured by standard addition method by diluting with TBA containing CoA standard resulting in 1 uM CoA standard addition. 1 uM CoA standard addition samples are denoted in the subject sample factor section.
Processing Method:Liquid extraction
Processing Storage Conditions:-80℃

Combined analysis:

Analysis ID AN002299
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Ascentis Express C18 (2.1 x 5 cm)
MS Type ESI
MS instrument type TOF
MS instrument name Waters Xevo-G2-XS
Ion Mode NEGATIVE
Units normalized signal area

Chromatography:

Chromatography ID:CH001689
Instrument Name:Waters Acquity
Column Name:Waters Ascentis Express C18 (2.1 x 5 cm)
Chromatography Type:Reversed phase

MS:

MS ID:MS002142
Analysis ID:AN002299
Instrument Name:Waters Xevo-G2-XS
Instrument Type:TOF
MS Type:ESI
MS Comments:Samples were run in MS^e mode. MassLynx V4.2 was used to measure area under the curve. Accurate mass and retention were used for identification of signal for respective metabolites.
Ion Mode:NEGATIVE
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