Summary of Study ST001387

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000951. The data can be accessed directly via it's Project DOI: 10.21228/M8RT2R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001387
Study TitleBat liver lipid profiles
Study SummaryIn this study, we investigated changes in hepatic lipid profiles of little brown bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus) at an early stage (70 d) of infection with the etiological agent, Pseudogymnoascus destructans (Pd).
Institute
Georgetown University
Last NamePannkuk
First NameEvan
Address3970 Reservoir Rd, NW New Research Build, washington dc, District of Columbia, 20057, USA
Emailelp44@georgetown.edu
Phone2026875650
Submit Date2020-06-01
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2021-08-31
Release Version1
Evan Pannkuk Evan Pannkuk
https://dx.doi.org/10.21228/M8RT2R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000951
Project DOI:doi: 10.21228/M8RT2R
Project Title:Bat liver lipid profiles
Project Summary:In this study, we investigated changes in hepatic lipid profiles of little brown bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus) at an early stage (70 d) of infection with the etiological agent, Pseudogymnoascus destructans (Pd).
Institute:Georgetown University
Last Name:Pannkuk
First Name:Evan
Address:3970 Reservoir Rd, NW New Research Build, washington dc, District of Columbia, 20057, USA
Email:elp44@georgetown.edu
Phone:2026875650

Subject:

Subject ID:SU001461
Subject Type:Mammal
Subject Species:Myotis lucifugus
Taxonomy ID:59463

Factors:

Subject type: Mammal; Subject species: Myotis lucifugus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA113100POS_37Pd
SA113101POS_38Pd
SA113102POS_35Pd
SA113103POS_33Pd
SA113104POS_10Pd
SA113105POS_42Pd
SA113106POS_43Pd
SA113107POS_53Pd
SA113108POS_52Pd
SA113109POS_48Pd
SA113110POS_46Pd
SA113111POS_30Pd
SA113112POS_32Pd
SA113113POS_18Pd
SA113114POS_13Pd
SA113115POS_23Pd
SA113116POS_11Pd
SA113117POS_12Pd
SA113118POS_15sham
SA113119POS_45sham
SA113120POS_47sham
SA113121POS_50sham
SA113122POS_51sham
SA113123POS_44sham
SA113124POS_49sham
SA113125POS_14sham
SA113126POS_17sham
SA113127POS_22sham
SA113128POS_31sham
SA113129POS_29sham
SA113130POS_28sham
SA113131POS_21sham
SA113132POS_34sham
SA113133POS_27sham
SA113134POS_19sham
SA113135POS_36sham
SA113136POS_20sham
SA113137POS_16sham
Showing results 1 to 38 of 38

Collection:

Collection ID:CO001456
Collection Summary:Laboratory experiments were conducted at the University of Winnipeg (Manitoba Sustainable Development Species at Risk/Wildlife Scientific Permit # SAR16009). M. lucifugus were collected from a WNS-negative hibernaculum in Central Manitoba in January (mid-winter) 2017. E. fuscus were collected from a WNS-negative hibernaculum in northwestern Ontario near Kenora in the same month. Hibernating bats were removed from the cave wall by hand and transferred to a bio-secure bat facility as previously described (Warnecke et al., 2012; McGuire et al., 2016). Bats were tested to be negative for Pd upon arrival using standard qPCR techniques and inoculated using previously established protocols (Warnecke et al., 2012; McGuire et al., 2016).
Sample Type:Liver

Treatment:

Treatment ID:TR001476
Treatment Summary:Bats given sham (PBST) or Pd inoculation

Sample Preparation:

Sampleprep ID:SP001469
Sampleprep Summary:Liver samples (~10 mg) were homogenized with cold methanol (300 μl) containing internal standards (10 μl SPLASH® Lipidomix®), 10 μl was removed for protein quantification, and the tissue homogenate was incubated on ice for 5 min. Chilled chloroform (600 μl) was added to samples and followed by shaking (vortex 30 sec) and further incubation on ice (10 min). Chilled water (300 μl) was added to samples followed by shaking (vortex 30 sec) and incubation on ice (10 min), then centrifuged for 10 min (10,000 x g, 4°C) to separate layers. The lower organic phase was separated, evaporated under vacuum, and reconstituted in 200 μl isopropanol (IPA):acetonitrile (ACN):H2O (2:1:1) for analysis.
Processing Storage Conditions:On ice
Extract Storage:On ice

Combined analysis:

Analysis ID AN002315 AN002316
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity CSH C18 (100 x 2.1mm,1.7um) Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 QTOF Waters Synapt G2 S QTOF
Ion Mode POSITIVE NEGATIVE
Units m/z values m/z values

Chromatography:

Chromatography ID:CH001702
Methods Filename:epannkuk_chromatography.docx
Instrument Name:Waters Acquity
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002158
Analysis ID:AN002315
Instrument Name:Waters Synapt G2 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:None
Ion Mode:POSITIVE
  
MS ID:MS002159
Analysis ID:AN002316
Instrument Name:Waters Synapt G2 S QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:None
Ion Mode:NEGATIVE
Analysis Protocol File:epannkuk_processing.docx
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