Summary of Study ST001623
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001041. The data can be accessed directly via it's Project DOI: 10.21228/M84M6M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001623 |
| Study Title | Targeted metabolomics analysis with raffinose-rich diet in mouse ileum |
| Study Type | Analysis of fructose level in ileum of stressed mouse fed with raffinose-rich diet |
| Study Summary | Purified diet (AIN-93G) supplemented with raffinose was prepared. The mice were maintained on the separate diet for at least 1 week before the initiation of experiment.Chronic restraint stress (RS) in mice was performed 14 days.After sacrifice, the ileum of mice were used to analysis. |
| Institute | China Pharmaceutical University |
| Department | School of Medicine |
| Laboratory | Metabonomics |
| Last Name | Hou |
| First Name | Yuanlong |
| Address | tongjiaxiang, nanjing, jiangsu, 210000, China |
| jian2103@163.com | |
| Phone | 18851105337 |
| Submit Date | 2020-12-09 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-12-16 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001041 |
| Project DOI: | doi: 10.21228/M84M6M |
| Project Title: | Dietary composition analysis studies |
| Project Type: | Untargeted metabolomics analysis with two diets |
| Project Summary: | Dietary patterns and psychosocial factors, ubiquitous part of modern lifestyle, critically shape the gut microbiota and human health. However, it remains obscure how dietary and psychosocial inputs coordinately modulate the gut microbiota and host impact. Here, we show that dietary raffinose metabolism to fructose couples stress-induced gut microbial remodeling to intestinal stem cells (ISC) renewal and epithelial homeostasis. Chow diet (CD) and purified diet (PD) confer distinct vulnerability to gut epithelial injury, microbial alternation and ISC dysfunction in chronically restrained mice. CD preferably enriches Lactobacillus reuteri, and its colonization is sufficient to rescue stress-triggered epithelial injury. |
| Institute: | China Pharmaceutical University |
| Department: | School of Medicine |
| Laboratory: | metabonomics |
| Last Name: | Yunalong |
| First Name: | Hou |
| Address: | Nanjing, Jiangsu |
| Email: | jian2103@163.com |
| Phone: | 18851105337 |
Subject:
| Subject ID: | SU001700 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA137927 | C2 | Con |
| SA137928 | C3 | Con |
| SA137929 | C1 | Con |
| SA137930 | C4 | Con |
| SA137931 | R6 | Raffinose |
| SA137932 | R7 | Raffinose |
| SA137933 | R8 | Raffinose |
| SA137934 | R5 | Raffinose |
| SA137935 | R4 | Raffinose |
| SA137936 | R3 | Raffinose |
| SA137937 | R1 | Raffinose |
| SA137938 | S2 | Stress |
| SA137939 | S3 | Stress |
| SA137940 | S1 | Stress |
| SA137941 | S4 | Stress |
| Showing results 1 to 15 of 15 |
Collection:
| Collection ID: | CO001693 |
| Collection Summary: | jejunum tissue (30 mg) was transferred to homogenizer (pre-cooled at -20 ℃) and 100 µL of H2O (pre-cooled at 4℃) was added for homogenate for 1 min. 1 μg/mL 4-chloro-phenylalanine and 800 µL methanol solution were added for protein precipitation and vortexed for 10 min. The sample lysates were centrifuged at 30000g for 10 min at 4 °C, and dried under vacuum. It was reconstituted with 100 μL of methanol: water solution (50:50, v/v) before analysis. |
| Sample Type: | Intestine |
Treatment:
| Treatment ID: | TR001713 |
| Treatment Summary: | PD was supplemented with raffinose, a significant increase in intestinal fructose in stressed mice could be observed. |
Sample Preparation:
| Sampleprep ID: | SP001706 |
| Sampleprep Summary: | Briefly, tissue (30 mg) was transferred to homogenizer (pre-cooled at -20 ℃) and 100 µL of H2O (pre-cooled at 4℃) was added for homogenate for 1 min. 1 μg/mL 4-chloro-phenylalanine and 800 µL methanol solution were added for protein precipitation and vortexed for 10 min. The sample lysates were centrifuged at 30000g for 10 min at 4 °C, and dried under vacuum. |
Combined analysis:
| Analysis ID | AN002658 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Waters Acquity H-Class |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Xevo G2-XS, Waters |
| Ion Mode | NEGATIVE |
| Units | relative peak area |
Chromatography:
| Chromatography ID: | CH001961 |
| Instrument Name: | Waters Acquity H-Class |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002465 |
| Analysis ID: | AN002658 |
| Instrument Name: | Xevo G2-XS, Waters |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS MassLynx v4.1 Quanlynx v4.1 |
| Ion Mode: | NEGATIVE |
