Summary of Study ST001648

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001054. The data can be accessed directly via it's Project DOI: 10.21228/M8FX20 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001648
Study Title	Comprehensive dissection of primary metabolites in response to diverse abiotic stress in barley at seedling stage
Study Type	Artical
Study Summary	Plants will meet various abiotic stresses during their growth and development. One of the important strategies for plants to deal with the stress is involved in metabolic regulation, causing the dramatic changes of metabolite profiles. Metabolomic studies have been intensively conducted to reveal the responses of plants to abiotic stress, but most of them were limited to one or at most two abiotic stresses in a single experiment. In this study, we compared the metabolite profiles of barley seedlings exposed to seven abiotic stresses simultaneously, including drought, salt stress, aluminum (Al), cadmium (Cd), deficiency of nitrogen (N), phosphorus (P) and potassium (K). The results showed that metabolite profiles of barley under these stresses could be classified into three types: osmotic stresses (drought and salt); metal stresses (Al and Cd) and nutrient deficiencies (N, P and K deficiencies). Compared with the control, some metabolites (including polyamines, raffinose and piperonic acid) in plants exposed to all abiotic stresses changed significantly, while some other metabolites showed the specific change only under a certain abiotic stress, such as proline being largely increased by osmotic stress (drought and salinity), the P-containing metabolites being largely decreased under P deficiency, some amino acids (lysine, tyrosine, threonine, ornithine， glutamine and so on) showing the dramatic reduction in the plants exposed to N deficiencies, respectively. The current meta-analysis obtained a comprehensive view on the metabolic responses to various abiotic stress, and improved the understanding of the mechanisms for tolerance of barley to abiotic stress.
Institute	Zhengjiang University
Department	Crop Research Institute
Laboratory	Barley Research Group of Crop Institute
Last Name	Zhao
First Name	Huifang
Address	West Lake District, 866 Yuhangtang Road
Email	11716028@zju.edu.cn
Phone	18888923662
Submit Date	2021-01-07
Num Groups	1
Total Subjects	1
Num Males	2
Num Females	1
Publications	PLANT PHYSIOLOGY AND BIOCHEMISTRY
Raw Data Available	Yes
Raw Data File Type(s)	d
Analysis Type Detail	API-MS
Release Date	2022-01-08
Release Version	1

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Project:

Project ID:	PR001054
Project DOI:	doi: 10.21228/M8FX20
Project Title:	Comprehensive dissection of primary metabolites in response to diverse abiotic stress in barley at seedling stage
Project Type:	Artical
Project Summary:	Plants will meet various abiotic stresses during their growth and development. One of the important strategies for plants to deal with the stress is involved in metabolic regulation, causing the dramatic changes of metabolite profiles. Metabolomic studies have been intensively conducted to reveal the responses of plants to abiotic stress, but most of them were limited to one or at most two abiotic stresses in a single experiment. In this study, we compared the metabolite profiles of barley seedlings exposed to seven abiotic stresses simultaneously, including drought, salt stress, aluminum (Al), cadmium (Cd), deficiency of nitrogen (N), phosphorus (P) and potassium (K). The results showed that metabolite profiles of barley under these stresses could be classified into three types: osmotic stresses (drought and salt); metal stresses (Al and Cd) and nutrient deficiencies (N, P and K deficiencies). Compared with the control, some metabolites (including polyamines, raffinose and piperonic acid) in plants exposed to all abiotic stresses changed significantly, while some other metabolites showed the specific change only under a certain abiotic stress, such as proline being largely increased by osmotic stress (drought and salinity), the P-containing metabolites being largely decreased under P deficiency, some amino acids (lysine, tyrosine, threonine, ornithine? glutamine and so on) showing the dramatic reduction in the plants exposed to N deficiencies, respectively. The current meta-analysis obtained a comprehensive view on the metabolic responses to various abiotic stress, and improved the understanding of the mechanisms for tolerance of barley to abiotic stress.
Institute:	Zhengjiang University
Department:	Crop Research Institute
Laboratory:	Barley Research Group of Crop Institute
Last Name:	Zhao
First Name:	Huifang
Address:	West Lake District, 866 Yuhangtang Road
Email:	11716028@zju.edu.cn
Phone:	18888923662
Funding Source:	National Key R&D Program of China; Natural Science Foundation of China; China Agriculture Research System
Publications:	PLANT PHYSIOLOGY AND BIOCHEMISTRY

Subject:

Subject ID:	SU001725
Subject Type:	Plant
Subject Species:	Hordeum vulgare L.
Taxonomy ID:	4513

Factors:

Subject type: Plant; Subject species: Hordeum vulgare L. (Factor headings shown in green)

mb_sample_id	local_sample_id	Treatment
SA151599	root-13	Root_Al-1
SA151600	root-14	Root_Al-2
SA151601	root-15	Root_Al-3
SA151602	root-16	Root_Al-4
SA151603	root-17	Root_Cd-1
SA151604	root-18	Root_Cd-2
SA151605	root-19	Root_Cd-3
SA151606	root-20	Root_Cd-4
SA151607	root-1	Root_Control-1
SA151608	root-2	Root_Control-2
SA151609	root-3	Root_Control-3
SA151610	root-4	Root_Control-4
SA151611	root-9	Root_Drought-1
SA151612	root-10	Root_Drought-2
SA151613	root-11	Root_Drought-3
SA151614	root-12	Root_Drought-4
SA151615	root-29	Root_K deficiency-1
SA151616	root-30	Root_K deficiency-2
SA151617	root-31	Root_K deficiency-3
SA151618	root-32	Root_K deficiency-4
SA151619	root-21	Root_N deficiency-1
SA151620	root-22	Root_N deficiency-2
SA151621	root-23	Root_N deficiency-3
SA151622	root-24	Root_N deficiency-4
SA151623	root-25	Root_P deficiency-1
SA151624	root-26	Root_P deficiency-2
SA151625	root-27	Root_P deficiency-3
SA151626	root-28	Root_P deficiency-4
SA151627	root-5	Root_Salt-1
SA151628	root-6	Root_Salt-2
SA151629	root-7	Root_Salt-3
SA151630	root-8	Root_Salt-4
SA151631	shoot-13	Shoot_Al-1
SA151632	shoot-14	Shoot_Al-2
SA151633	shoot-15	Shoot_Al-3
SA151634	shoot-16	Shoot_Al-4
SA151635	shoot-17	Shoot_Cd-1
SA151636	shoot-18	Shoot_Cd-2
SA151637	shoot-19	Shoot_Cd-3
SA151638	shoot-20	Shoot_Cd-4
SA151639	shoot-1	Shoot_Control-1
SA151640	shoot-2	Shoot_Control-2
SA151641	shoot-3	Shoot_Control-3
SA151642	shoot-4	Shoot_Control-4
SA151643	shoot-9	Shoot_Drought-1
SA151644	shoot-10	Shoot_Drought-2
SA151645	shoot-11	Shoot_Drought-3
SA151646	shoot-12	Shoot_Drought-4
SA151647	shoot-29	Shoot_K deficiency-1
SA151648	shoot-30	Shoot_K deficiency-2
SA151649	shoot-31	Shoot_K deficiency-3
SA151650	shoot-32	Shoot_K deficiency-4
SA151651	shoot-21	Shoot_N deficiency-1
SA151652	shoot-22	Shoot_N deficiency-2
SA151653	shoot-23	Shoot_N deficiency-3
SA151654	shoot-24	Shoot_N deficiency-4
SA151655	shoot-25	Shoot_P deficiency-1
SA151656	shoot-26	Shoot_P deficiency-2
SA151657	shoot-27	Shoot_P deficiency-3
SA151658	shoot-28	Shoot_P deficiency-4
SA151659	shoot-5	Shoot_Salt-1
SA151660	shoot-6	Shoot_Salt-2
SA151661	shoot-7	Shoot_Salt-3
SA151662	shoot-8	Shoot_Salt-4

Showing results 1 to 64 of 64

Showing results 1 to 64 of 64

Collection:

Collection ID:	CO001718
Collection Summary:	Barley seedlings were allowed to grow up to two-leaf-stage in the normal hydroponic solution, and then exposed to various treatments after removing endosperm for eliminating its nutrient supply. The treatments included 20% PEG for drought stress, 150 mM NaCl for salt stress, 50 μM Al3+ at pH 4.5 for aluminum stress (Al stress, at the same time the nutrient solution with pH 4.5 and without Al addition used as control), 5 μM CdCl2 for cadmium stress (Cd stress), 0 mM N for N deficiency (replace KNO3, Ca(NO3).4H2O and (NH4)6Mo7O24 with 1mM CaCl2, 1mM KH2PO4 and 0.07 mM H2MoO4), 0 mM P for P deficiency (replace NH4H2PO4 with 1mM NH4Cl), 0 mM K for K deficiency (replace KNO3 with 1mM NaNO3). The hydroponic solution was renewed every four days. After twenty days of the treatments, the plants were harvested, and fresh and dry weight were recorded quickly. The dry tissues were used for mineral element concentration analysis with three biological replicates for each treatment. Meanwhile, fresh tissues were frozen in liquid nitrogen and stored at -80 ℃ for metabolite analysis with four biological replicates for each stress treatment.
Sample Type:	Plant
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR001738
Treatment Summary:	The treatments included 20% PEG for drought stress, 150 mM NaCl for salt stress, 50 μM Al3+ at pH 4.5 for aluminum stress (Al stress, at the same time the nutrient solution with pH 4.5 and without Al addition used as control), 5 μM CdCl2 for cadmium stress (Cd stress), 0 mM N for N deficiency (replace KNO3, Ca(NO3).4H2O and (NH4)6Mo7O24 with 1mM CaCl2, 1mM KH2PO4 and 0.07 mM H2MoO4), 0 mM P for P deficiency (replace NH4H2PO4 with 1mM NH4Cl), 0 mM K for K deficiency (replace KNO3 with 1mM NaNO3).

Treatment ID:

TR001738

Treatment Summary:

The treatments included 20% PEG for drought stress, 150 mM NaCl for salt stress, 50 μM Al3+ at pH 4.5 for aluminum stress (Al stress, at the same time the nutrient solution with pH 4.5 and without Al addition used as control), 5 μM CdCl2 for cadmium stress (Cd stress), 0 mM N for N deficiency (replace KNO3, Ca(NO3).4H2O and (NH4)6Mo7O24 with 1mM CaCl2, 1mM KH2PO4 and 0.07 mM H2MoO4), 0 mM P for P deficiency (replace NH4H2PO4 with 1mM NH4Cl), 0 mM K for K deficiency (replace KNO3 with 1mM NaNO3).

Sample Preparation:

Sampleprep ID:	SP001731
Sampleprep Summary:	The frozen tissue was homogenized in liquid nitrogen and approximately 100 mg of powder sample was added into 2 ml vertical centrifuge tube. Then 1.4 ml of 100% methanol (pre-cooled at -20 °C) was added and vortexed thoroughly. Then the tubes were shaken for 10 min at 70 °C, followed by centrifugation at 11,000 g for 10 min. The supernatant was transferred into a new 10 ml sterile centrifuge tube, and 750 μl chloroform (−20 °C) and 1.5 ml deionized water (4 °C) were added. The mixture was vortexed and centrifuged at 2200 g for 15 min. The upper methanol/water phase was collected for metabolite analysis. A total of 150 μl collected solution was dried in a vacuum freeze dryer. The dried residue was derivatized in 40 μl of 15 mg ml-1 methoxylamine hydrochloride in pyridine for 120 min at 37 °C, followed by reaction with 70 μl of N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) reagent for another 30 min at 37 °C. After the reaction, the reacted solution was transferred to glass vial insert in 2 ml glass bottle (Agilent, USA) for GC-MS determination. Meanwhile, one blank derivatization reaction (with reagent) was also prepared as a control.

Sampleprep ID:

SP001731

Sampleprep Summary:

The frozen tissue was homogenized in liquid nitrogen and approximately 100 mg of powder sample was added into 2 ml vertical centrifuge tube. Then 1.4 ml of 100% methanol (pre-cooled at -20 °C) was added and vortexed thoroughly. Then the tubes were shaken for 10 min at 70 °C, followed by centrifugation at 11,000 g for 10 min. The supernatant was transferred into a new 10 ml sterile centrifuge tube, and 750 μl chloroform (−20 °C) and 1.5 ml deionized water (4 °C) were added. The mixture was vortexed and centrifuged at 2200 g for 15 min. The upper methanol/water phase was collected for metabolite analysis. A total of 150 μl collected solution was dried in a vacuum freeze dryer. The dried residue was derivatized in 40 μl of 15 mg ml-1 methoxylamine hydrochloride in pyridine for 120 min at 37 °C, followed by reaction with 70 μl of N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) reagent for another 30 min at 37 °C. After the reaction, the reacted solution was transferred to glass vial insert in 2 ml glass bottle (Agilent, USA) for GC-MS determination. Meanwhile, one blank derivatization reaction (with reagent) was also prepared as a control.

Combined analysis:

Analysis ID	AN002694
Analysis type	MS
Chromatography type	GC
Chromatography system	Agilent 7890A
Column	Agilent DB5-MS (30m x 0.25mm, 0.25um)
MS Type	API
MS instrument type	GC-TOF
MS instrument name	Agilent 7890A
Ion Mode	UNSPECIFIED
Units	pmoles/l

Chromatography:

Chromatography ID:	CH001987
Instrument Name:	Agilent 7890A
Column Name:	Agilent DB5-MS (30m x 0.25mm, 0.25um)
Chromatography Type:	GC

MS:

MS ID:	MS002492
Analysis ID:	AN002694
Instrument Name:	Agilent 7890A
Instrument Type:	GC-TOF
MS Type:	API
MS Comments:	The retention time (RT) and mass spectra of each compound were analyzed using AMDIS (Automated Mass Spectral Deconvolution and Identification System) software (http://chemdata.nist.gov/).
Ion Mode:	UNSPECIFIED