Summary of study ST001670

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001073. The data can be accessed directly via it's Project DOI: 10.21228/M80Q4N This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001670
Study TitleMetabolomics characterization of zebrafish larvae
Study TypeMS analysis
Study SummaryFour different treatment groups were used for metabolite characterization: 5 dpf larvae with/without beta-cell ablation and with/without folinic acid treatment.
North Carolina State University
DepartmentMolecular and Structural Biochemistry
Last NameDuan
First NameLikun
Address120 W Broughton Dr.
Submit Date2021-01-15
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2021-03-15
Release Version1
Likun Duan Likun Duan application/zip

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Project ID:PR001073
Project DOI:doi: 10.21228/M80Q4N
Project Title:Reinforcing one-carbon metabolism via folic acid/Folr1 promotes beta-cell formation
Project Summary:Diabetes can be caused by an insufficient beta-cell mass. Here, we performed a genetic screen in a zebrafish model with beta-cell loss to identify pathways promoting beta-cell regeneration. We found that both folate receptor 1 (folr1) overexpression and treatment with folate intermediates, i.e. tetrahydrofolic acid (THF) or folinic acid stimulated beta-cell differentiation in zebrafish. Treatment with these folate intermediates also stimulated beta-cell differentiation in neonatal pig islet cultures, showing the effect could be translated to a mammalian system. In both zebrafish and neonatal pig islets, the increased beta-cell differentiation originated from ductal cells. Mechanistically, comparative metabolomics analyses of zebrafish in the basal state vs following -cell ablation and in un-treated vs folinic acid-treated zebrafish indicated beta-cell regeneration could be attributed to changes in the pyrimidine, carnitine and serine pathways. Overall, our results suggest evolutionary conserved and previously unknown roles of folic acid and one-carbon metabolism in the generation of beta-cell .
Institute:North Carolina State University
Department:Molecular and Structural Biochemistry
Last Name:Duan
First Name:Likun
Address:120 W Broughton Dr.