Summary of study ST001670

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001073. The data can be accessed directly via it's Project DOI: 10.21228/M80Q4N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001670
Study TitleMetabolomics characterization of zebrafish larvae
Study TypeMS analysis
Study SummaryFour different treatment groups were used for metabolite characterization: 5 dpf larvae with/without beta-cell ablation and with/without folinic acid treatment.
Institute
North Carolina State University
DepartmentMolecular and Structural Biochemistry
Last NameDuan
First NameLikun
Address120 W Broughton Dr.
Emaillduan2@ncsu.edu
Phone9195939853
Submit Date2021-01-15
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2021-03-15
Release Version1
Likun Duan Likun Duan
https://dx.doi.org/10.21228/M80Q4N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001073
Project DOI:doi: 10.21228/M80Q4N
Project Title:Reinforcing one-carbon metabolism via folic acid/Folr1 promotes beta-cell formation
Project Summary:Diabetes can be caused by an insufficient beta-cell mass. Here, we performed a genetic screen in a zebrafish model with beta-cell loss to identify pathways promoting beta-cell regeneration. We found that both folate receptor 1 (folr1) overexpression and treatment with folate intermediates, i.e. tetrahydrofolic acid (THF) or folinic acid stimulated beta-cell differentiation in zebrafish. Treatment with these folate intermediates also stimulated beta-cell differentiation in neonatal pig islet cultures, showing the effect could be translated to a mammalian system. In both zebrafish and neonatal pig islets, the increased beta-cell differentiation originated from ductal cells. Mechanistically, comparative metabolomics analyses of zebrafish in the basal state vs following -cell ablation and in un-treated vs folinic acid-treated zebrafish indicated beta-cell regeneration could be attributed to changes in the pyrimidine, carnitine and serine pathways. Overall, our results suggest evolutionary conserved and previously unknown roles of folic acid and one-carbon metabolism in the generation of beta-cell .
Institute:North Carolina State University
Department:Molecular and Structural Biochemistry
Last Name:Duan
First Name:Likun
Address:120 W Broughton Dr.
Email:lduan2@ncsu.edu
Phone:9195939853
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