Summary of Study ST001686

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001084. The data can be accessed directly via it's Project DOI: 10.21228/M8KH57 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001686
Study TitleExposure to per- and polyfluoroalkyl substances associates with altered lipid profile of breast milk (Part 3)
Study SummaryIn this mother-infant study (n=44) we investigated the levels of PFAS in maternal serum and detailed lipidomic profile in breast milk at birth and at three months using ultra high performance liquid chromatography combined with quadrupole-time-of-flight mass spectrometry.
Institute
University of Turku
Last NameLamichhane
First NameSantosh
AddressTykistökatu 6, FI-20520 Turku, Finland
Emailsantosh.lamichhane@utu.fi
Phone0452299070
Submit Date2021-02-08
Raw Data AvailableYes
Raw Data File Type(s)mzdata.xml
Analysis Type DetailLC-MS
Release Date2021-10-02
Release Version1
Santosh Lamichhane Santosh Lamichhane
https://dx.doi.org/10.21228/M8KH57
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001084
Project DOI:doi: 10.21228/M8KH57
Project Title:Exposure to per- and polyfluoroalkyl substances and lipid profile of breast milk
Project Type:MS analysis
Project Summary:The objective of this study was to investigate whether the maternal levels of PFAS are associated with lipid composition of human breast milk and further, if the changes in composition have impact on the growth of the infants.
Institute:University of Turku
Department:Turku Bioscience
Laboratory:Turku Metabolomics Center
Last Name:Lamichhane
First Name:Santosh
Address:Tykistökatu 6 , Turku
Email:santosh.lamichhane@utu.fi
Phone:0452299070
Funding Source:This study was supported by the Swedish Research Council (grant no. 2016-05176 to T.H and M.O), Formas (grant no. 2019-00869 to T.H and M.O), and the NovoNordiskFoundation (xxx). The EDIA study was supported by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (No. 1DP3DK094338-01 to M.K.), the Academy of Finland Centre of Excellence in Molecular Systems Immunology and Physiology Research 2012-17, No. 250114 to M.K. and M.O.). Further support was received by the Academy of Finland postdoctoral grant (No. 323171 to S.L.) and the Medical Research Funds, Tampere and Helsinki University Hospitals (to M.K.).
Project Comments:Parts 1, 2 and 3
Contributors:Santosh Lamichhane, Heli Siljander, Daniel Duberg, Jorma Ilonen, Suvi M. Virtanen, Matej Oreši?, Mikael Knip, Tuulia Hyötyläinen

Subject:

Subject ID:SU001763
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Not applicable

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Code
SA155453744 _23 months
SA155454757 _23 months
SA155455764 _23 months
SA155456740 _23 months
SA155457673 _23 months
SA155458514 _23 months
SA155459568 _23 months
SA155460776 _23 months
SA155461739 _23 months
SA155462792 _23 months
SA1554631294 _23 months
SA1554641300 _23 months
SA1554651348 _23 months
SA155466969 _23 months
SA155467820 _23 months
SA155468806 _23 months
SA155469808 _23 months
SA155470510 _23 months
SA155471487 _23 months
SA155472226 _23 months
SA155473259 _23 months
SA155474297 _23 months
SA155475201 _23 months
SA15547684 _23 months
SA15547759 _23 months
SA15547872 _23 months
SA15547974 _23 months
SA155480305 _23 months
SA155481327 _23 months
SA155482427 _23 months
SA155483450 _23 months
SA155484471 _23 months
SA155485399 _23 months
SA155486369 _23 months
SA155487333 _23 months
SA155488346 _23 months
SA155489353 _23 months
SA15549018 _23 months
Showing results 1 to 38 of 38

Collection:

Collection ID:CO001756
Collection Summary:Blood samples collected for this study were allowed to clot at room temperature. The clot was removed by centrifugation and the resulting supernatant serum samples were stored at −80 °C until analyzed.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR001776
Treatment Summary:Sample were stored at -80 as it is, no treatment done.

Sample Preparation:

Sampleprep ID:SP001769
Sampleprep Summary:10 µl of serum of was extracted with using a modified version of the previously published Folch procedure. In short, 10 µL of 0.9% NaCl and, 120 µL of CHCl3: MeOH (2:1, v/v) containing the internal standards (c = 2.5 µg/mL) was added to the sample. The standard solution contained the following compounds: 1,2-diheptadecanoyl-sn-glycero-3-phosphoethanolamine (PE(17:0/17:0)), N-heptadecanoyl-D-erythro-sphingosylphosphorylcholine (SM(d18:1/17:0)), N-heptadecanoyl-D-erythro-sphingosine (Cer(d18:1/17:0)), 1,2-diheptadecanoyl-sn-glycero-3-phosphocholine (PC(17:0/17:0)), 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC(17:0)) and 1-palmitoyl-d31-2-oleoyl-sn-glycero-3-phosphocholine (PC(16:0/d31/18:1)), were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA), and, triheptadecanoylglycerol (TG(17:0/17:0/17:0)) was purchased from Larodan AB (Solna, Sweden). The samples were vortex mixed and incubated on ice for 30 min after which they were centrifuged (9400 × g, 3 min). 60 µL from the lower layer of each sample was then transferred to a glass vial with an insert and 60 µL of CHCl3: MeOH (2:1, v/v) was added to each sample. The samples were stored at -80 °C until analysis.
Processing Storage Conditions:-80℃

Combined analysis:

Analysis ID AN002753
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity UPLC
Column BEH C18
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6210 TOF
Ion Mode NEGATIVE
Units ng/ml

Chromatography:

Chromatography ID:CH002034
Chromatography Summary:Column name, BEH C18 (2.1 x 100 mm, particle size 1.7 µm) (Waters Corporation, Milford, MA, USA)
Instrument Name:Waters Acquity UPLC
Column Name:BEH C18
Chromatography Type:Reversed phase

MS:

MS ID:MS002550
Analysis ID:AN002753
Instrument Name:Agilent 6210 TOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Dual jet stream electrospray (dual ESI) ion source was used and the ion polarity was on negative mode. The capillary voltage and the nozzle voltage were kept at 4500 V and 1500 V. The N2 pressure was set on 21 psi, with the sheath gas flow as 11 L/min and temperature at 379°C for the nebulizer. The data was acquired with MassHunter B.06.01 software (Agilent Technologies, Santa Clara, CA, The United States of America).
Ion Mode:NEGATIVE
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