Summary of Study ST001754
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001115. The data can be accessed directly via it's Project DOI: 10.21228/M8KD8B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001754 |
| Study Title | Metabolomic Analysis of Canine Diabetes (part-II) |
| Study Type | Single time point blood collection |
| Study Summary | Blood was collected from diabetic dogs and healthy control dogs. Diabetic dogs and control dogs were breed matched where possible. Timing of blood collection after a meal was matched as best as posible. Serum was frozen at -80C. |
| Institute | University of Florida |
| Last Name | O'Kell |
| First Name | Allison |
| Address | 2015 SW 16th Ave |
| aokell@ufl.edu | |
| Phone | 352-294-4471 |
| Submit Date | 2021-04-01 |
| Num Groups | 2 |
| Total Subjects | 30 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-10-29 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001115 |
| Project DOI: | doi: 10.21228/M8KD8B |
| Project Title: | Utility of canine diabetes as a model for human type 1 diabetes (part-I) |
| Project Summary: | We are examining the etiopathogenesis of canine diabetes to determine the relevance of these animals as a naturally occurring model of human type 1 diabetes. We will undertake phenotypic, metabolic, and immunologic investigations in diabetic dogs to better characterize their diabetes and attempt to identify potential biomarkers of a pre-diabetic state. |
| Institute: | University of Florida |
| Department: | Diabetes Institute (Wasserfall/Atkinson)/College of Veterinary Medicine (O'Kell) |
| Last Name: | O'Kell |
| First Name: | Allison |
| Address: | 2015 SW 16th Ave |
| Email: | aokell@ufl.edu |
| Phone: | 352-294-4471 |
Subject:
| Subject ID: | SU001831 |
| Subject Type: | Mammal |
| Subject Species: | Canis lupus familiaris |
| Taxonomy ID: | 9615 |
Factors:
Subject type: Mammal; Subject species: Canis lupus familiaris (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diabetic or Healthy | Sex | Fasted or Fed | Breed |
|---|---|---|---|---|---|
| SA163962 | 327679 | Diabetic | MI | Fed | Labrador retriever |
| SA163963 | 325419 | Diabetic | MN | Fasted | Bichon Frise |
| SA163964 | 354675 | Diabetic | MN | Fasted | Maltese |
| SA163965 | 346336 | Diabetic | MN | Fasted | Mix (Jack Russell Terrier) |
| SA163966 | 349833 | Diabetic | MN | Fasted | Pomeranian |
| SA163967 | 353791 | Diabetic | MN | Fasted | Silky Terrier |
| SA163968 | 319373 | Diabetic | MN | Fed | Cavalier King Charles Spaniel |
| SA163969 | 308098 | Diabetic | MN | Fed | Miniature Australian Shepherd |
| SA163970 | 333987 | Diabetic | MN | Fed | Mix (Pug/beagle) |
| SA163971 | 348332 | Diabetic | MN | Fed | Rottweiler |
| SA163972 | 313074 | Diabetic | SF | Fasted | Miniature Pinscher |
| SA163973 | 329649 | Diabetic | SF | Fasted | Mix |
| SA163974 | 355594 | Diabetic | SF | Fasted | Mix |
| SA163975 | 349937 | Diabetic | SF | Fasted | Rottweiler |
| SA163976 | 242045 | Diabetic | SF | Fed | Miniature Schnauzer |
| SA163977 | 301154 | Healthy | MN | Fasted | Maltese mix |
| SA163978 | 309999 | Healthy | MN | Fasted | Rottweiler |
| SA163979 | 280189 | Healthy | MN | Fed | Labrador retriever |
| SA163980 | 328335 | Healthy | MN | Fed | Miniature Australian Shepherd |
| SA163981 | 232368 | Healthy | MN | Fed | Mix (Beagle) |
| SA163982 | 295509 | Healthy | MN | Fed | Rottweiler |
| SA163983 | 292894 | Healthy | SF | Fasted | Bichon/Poodle |
| SA163984 | 344481 | Healthy | SF | Fasted | Miniature Pinscher |
| SA163985 | 243765 | Healthy | SF | Fasted | Mix |
| SA163986 | 355837 | Healthy | SF | Fasted | Mix |
| SA163987 | 298006 | Healthy | SF | Fasted | Mix (Jack Russell Terrier) |
| SA163988 | 360399 | Healthy | SF | Fasted | Pomeranian |
| SA163989 | 359130 | Healthy | SF | Fasted | Silky Terrier |
| SA163990 | 277908 | Healthy | SF | Fed | Cavalier King Charles Spaniel |
| SA163991 | 274821 | Healthy | SF | Fed | Miniature Schnauzer |
| Showing results 1 to 30 of 30 |
Collection:
| Collection ID: | CO001824 |
| Collection Summary: | Blood collected by peripheral venipuncture into red top tube (containing clot activator). Blood was allowed to clot, then centrifuged at 2000 g x 7.5 minutes between 20-30 minutes after sample collection. Serum was frozen immediately after separation (always within 1 hour of collection) at -80 C. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR001844 |
| Treatment Summary: | None for research study. Variable medications may have been received by diabetic dogs. Healthy dogs are only allowed to receive flea/tick/heartworm preventatives. |
Sample Preparation:
| Sampleprep ID: | SP001837 |
| Sampleprep Summary: | see protocol attached |
| Sampleprep Protocol Filename: | Protein_Precipitation_Sample_Prep.pdf |
Combined analysis:
| Analysis ID | AN002857 | AN002858 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Exactive Plus Orbitrap | Thermo Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Height | Peak Height |
Chromatography:
| Chromatography ID: | CH002116 |
| Chromatography Summary: | see attached |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002650 |
| Analysis ID: | AN002857 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | see attached |
| Ion Mode: | POSITIVE |
| MS ID: | MS002651 |
| Analysis ID: | AN002858 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | see attached |
| Ion Mode: | NEGATIVE |
