Summary of Study ST001846

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001163. The data can be accessed directly via it's Project DOI: 10.21228/M8CH74 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001846
Study TitleProteasome Inhibitor Related Cardiotoxicity (part I)
Study SummaryBALB/c mice were injected with CFZ (16 mg/kg, iv), BTZ (1 mg/kg, iv), CFZ/atRA (CFZ: 16 mg/kg iv, atRA: 1 mg/kg iv) or Captisol (Vehicle, iv) (6 mice per cohort). ECGs were recorded under anesthesia at the end of 1h treatment and the mice were sacrificed by cervical dislocation. Hearts were extracted and were frozen and stored at -80°C until analysis
Institute
University of Zurich
Last NameMendez
First NameMax
AddressRorschacherstrasse 95. 9007, St.Gallen
Emailmaxmendez@runbox.com
Phone+41 71 494 10 67
Submit Date2021-06-01
Num Groups4
Total Subjects24
Num Females24
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-12-01
Release Version1
Max Mendez Max Mendez
https://dx.doi.org/10.21228/M8CH74
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001163
Project DOI:doi: 10.21228/M8CH74
Project Title:Proteasome Inhibitor Related Cardiotoxicity
Project Type:MS Untargeted Metabolomics from Murine Hearts
Project Summary:In this study we aimed to examine the acute metabolomic changes following proteasome inhibition with Bortezomib or Carfilzomib in murine heart.
Institute:University of Zurich
Department:Department of Hematology-Oncology. Kantonsspital St. Gallen
Laboratory:Experimental Hematolgy-KSSG
Last Name:Mendez
First Name:Max
Address:Rorschacherstrasse 95, 9007, St. Gallen.
Email:maxmendez@runbox.com
Phone:+41 71 494 10 67

Subject:

Subject ID:SU001923
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:Balb/c
Age Or Age Range:8-12 weeks old
Weight Or Weight Range:20-25 g
Gender:Female
Animal Feed:ad libitum
Animal Water:ad libitum

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id class
SA171686tCFZ3_POScaseB
SA171687tCFZ6_POScaseB
SA171688tCFZ4_NEGcaseB
SA171689tCFZ5_POScaseB
SA171690tCFZ1_POScaseB
SA171691tCFZ4_POScaseB
SA171692tCFZ2_NEGcaseB
SA171693tCFZ6_NEGcaseB
SA171694tCFZ5_NEGcaseB
SA171695tCFZ3_NEGcaseB
SA171696tCFZ1_NEGcaseB
SA171697tCFZ2_POScaseB
SA171698tCFZ.ATRA3_POScaseC
SA171699tCFZ.ATRA3_NEGcaseC
SA171700tCFZ.ATRA1_POScaseC
SA171701tCFZ.ATRA4_POScaseC
SA171702tCFZ.ATRA2_POScaseC
SA171703tCFZ.ATRA5_POScaseC
SA171704tCFZ.ATRA6_POScaseC
SA171705tCFZ.ATRA5_NEGcaseC
SA171706tCFZ.ATRA4_NEGcaseC
SA171707tCFZ.ATRA1_NEGcaseC
SA171708tCFZ.ATRA6_NEGcaseC
SA171709tCFZ.ATRA2_NEGcaseC
SA171710tBTZ4_POScaseD
SA171711tBTZ3_POScaseD
SA171712tBTZ6_POScaseD
SA171713tBTZ5_POScaseD
SA171714tBTZ1_POScaseD
SA171715tBTZ6_NEGcaseD
SA171716tBTZ5_NEGcaseD
SA171717tBTZ4_NEGcaseD
SA171718tBTZ.3_NEGcaseD
SA171719tBTZ1_NEGcaseD
SA171720tBTZ2_NEGcaseD
SA171721tBTZ2_POScaseD
SA171722tCTRL2_POScontrol
SA171723tCTRL5_NEGcontrol
SA171724tCTRL4_NEGcontrol
SA171725tCTRL6_NEGcontrol
SA171726tCTRL4_POScontrol
SA171727tCTRL1_POScontrol
SA171728tCTRL1_NEGcontrol
SA171729tCTRL2_NEGcontrol
SA171730tCTRL3_POScontrol
SA171731tCTRL3_NEGcontrol
SA171732tCTRL6_POScontrol
SA171733tCTRL5_POScontrol
SA171666QC_07_POSQC
SA171667QC_08_POSQC
SA171668QC_06_NEGQC
SA171669QC_14_NEGQC
SA171670QC_13_NEGQC
SA171671QC_09_POSQC
SA171672QC_15_NEGQC
SA171673QC_10_POSQC
SA171674QC_14_POSQC
SA171675QC_15_POSQC
SA171676QC_13_POSQC
SA171677QC_12_POSQC
SA171678QC_11_POSQC
SA171679QC_12_NEGQC
SA171680QC_06_POSQC
SA171681QC_10_NEGQC
SA171682QC_09_NEGQC
SA171683QC_11_NEGQC
SA171684QC_07_NEGQC
SA171685QC_08_NEGQC
Showing results 1 to 68 of 68

Collection:

Collection ID:CO001916
Collection Summary:Anesthesia was induced with Ketamine 80 mg/kg intramuscular and maintained with Isoflurane 0.5%. Hearts were extracted immediately after cervical dislocation under anesthesia, snap frozen in dry ice and kept at -80°C until analysis.
Sample Type:Cardiac tissue
Storage Conditions:Described in summary

Treatment:

Treatment ID:TR001935
Treatment Summary:Injections with CFZ (16 mg/kg, iv), BTZ (1 mg/kg, iv), CFZ+atRA (CFZ: 16 mg/kg iv, atRA: 1 mg/kg iv) or Captisol (Vehicle, iv)
Treatment Compound:Bortezomib (PS-34, Selleck Chemicals, S1013); Carfilzomib (PR-171, Selleck Chemicals, S2853); all-trans-Retinoic-Acid (Sigma-Aldrich, R2625); Captisol (Ligand Pharmaceuticals
Treatment Route:i.v
Treatment Dose:As described in treatment summary
Treatment Vehicle:Captisol
Animal Anesthesia:Induction with Ketamine 80 mg/kg intramuscular and maintained with Isoflurane 0.5%
Animal Endp Euthanasia:1h
Animal Endp Tissue Coll List:1h

Sample Preparation:

Sampleprep ID:SP001929
Sampleprep Summary:1 Take 100 μL of each sample (including QC) into the corresponding 96-well plate; 2 Add 400 μL of precipitant (methanol: acetonitrile = 2:1, V/V) to the sample, seal the membrane with a film sealer, and shake for 1 min; 3 Place the sample in ice water for ultrasonication for 10 min; 4 Place the sonicated sample at -20°C for 2h; 5 Centrifuge the sample in a centrifuge (4,000 rpm * 4°C, 30 min), each sample taken 300 μL to the new 96-well plate; 6 Put the 96-well plate into a freeze dryer and drain it; 7 On the drained plate, add 300 μL of 10% methanol, mix with a Multi-Tube vorterer (parameter setting 2,500HZ, 3 min), and place in ice water for 10 min; 8 Centrifuge for 20 min (4,000 rpm * 4°C), take 250 μL of the supernatant on a new plate; 9 For each sample (excluding preparation QC), take 50 μL of each sample and mix into a QC sample, and the prepared supernatant is ready to be used.
Sampleprep Protocol Filename:MetaboExtract_en.txt

Combined analysis:

Analysis ID AN002989 AN002990
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Xevo G2 XS QTOF Waters Xevo G2 XS QTOF
Ion Mode POSITIVE NEGATIVE
Units Peak Area Peak Area

Chromatography:

Chromatography ID:CH002218
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002779
Analysis ID:AN002989
Instrument Name:Waters Xevo G2 XS QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Capillary and sampling cone voltages were set at 3.0kV and 40.0V respectively. The mass spectrometry data were acquired in Centroid MSE mode. The TOF mass range was from 50-1200Da and the scan time was 0.2s. Peak Extraction and identification was performed with Progenesis QI (version2.2)
Ion Mode:POSITIVE
  
MS ID:MS002780
Analysis ID:AN002990
Instrument Name:Waters Xevo G2 XS QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Capillary and sampling cone voltages were set at 2.0kV and 40.0V respectively. The mass spectrometry data were acquired in Centroid MSE mode. The TOF mass range was from 50-1200Da and the scan time was 0.2s. Peak Extraction and identification was performed with Progenesis QI (version2.2)
Ion Mode:NEGATIVE
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