Summary of Study ST001871

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001181. The data can be accessed directly via it's Project DOI: 10.21228/M82418 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001871
Study TitleUntargeted LC-MS metabolomics analysis of cecal content of mice treated with TCDD vs. vehicle control (part I)
Study TypeUntargeted metabolomics
Study SummarySix-week-old female wildtype (WT) C57BL/6 mice were administered a single 100µl intraperitoneal injection containing sterile corn oil (VEH group) or an intraperitoneal injection of 10µg/kg TCDD suspended within sterile corn oil (TCDD group). At the 72h time point following TCDD or VEH exposure, the mice were humanely euthanized by an overdose of inhaled isoflurane. During necropsy, cecal content and blood serum samples were collected for untargeted metabolomics profiling.
Institute
University of South Carolina School of Medicine
DepartmentDepartment of Pathology
Laboratory(On behalf of) Mitzi Nagarkatti Lab
Last NameLai
First NameYunjia
Address135 Dauer Drive, Chapel Hill, NC 27599
Emailyunjia.lai@outlook.com
Phone919-480-5489
Submit Date2021-07-16
Num Groups2
Total Subjects20
Num Females20
PublicationsChemosphere
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-08-30
Release Version1
Yunjia Lai Yunjia Lai
https://dx.doi.org/10.21228/M82418
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001181
Project DOI:doi: 10.21228/M82418
Project Title:Effects of Acute 2,3,7,8-Tetrachlorodibenzo-p-dioxin Exposure on the Circulating and Cecal Metabolome Profile
Project Type:Untargeted metabolomics
Project Summary:2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a polyhalogenated planar hydrocarbon belonging to a group of highly toxic and persistent environmental contaminants known as “dioxins”. TCDD is an animal teratogen and carcinogen that is well characterized for causing immunosuppression through activation of Aryl Hydrocarbon Receptor (AHR). In the current study, we investigated the effect of exposure of mice to an acute dose of TCDD on the metabolic profile within the serum and cecal contents to better define the effects of TCDD on host physiology. Our findings demonstrated that within the circulating metabolome following acute TCDD, there was significant dysregulation in the metabolism of bioactive lipids, amino acids, and carbohydrates when compared to the VEH treated mice. These wide-spread changes in metabolite abundance were identified to regulate host immunity via modulating Nuclear Factor-Kappa B (NF-κB) and Extracellular Signal‑Regulated Protein Kinase (ERK1/2) activity, and work as biomarkers for a variety of organ injuries and dysfunctions that follow TCDD exposure. Within the cecal content, of mice exposed to TCDD, we were able to detect changes in inflammatory markers that regulate NF-κB, markers of injury-related inflammation, and changes in lysine degradation, nicotinamide metabolism, and butanoate metabolism, which suggested an immediate suppression microbial metabolism. Collectively, these results demonstrate that acute TCDD exposure results in immediate irregularities in the circulating and intestinal metabolome which likely contributes to TCDD toxicity and can be used as biomarkers for the early detection of individual exposure.
Institute:University of South Carolina School of Medicine
Department:Department of Pathology
Laboratory:On behalf of Dr. Mitzi Nagarkatti Lab
Last Name:Lai
First Name:Yunjia
Address:1104 MHRC, 135 Dauer Dr., Chapel Hill, NC, 27599, USA
Email:yunjia.lai@outlook.com
Phone:9194805489
Funding Source:National Institute of Health: P01AT003961, P20GM103641, R01ES030144, R01AI129788 and R01AI123947
Project Comments:This is supporting metabolomics data for the publication in Chemosphere.
Publications:Chemosphere

Subject:

Subject ID:SU001948
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6 mice (Wild-type)
Age Or Age Range:six weeks old
Gender:Female

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Experimental factor
SA174745CT5TCDD
SA174746CT4TCDD
SA174747CT3TCDD
SA174748CT6TCDD
SA174749CT8TCDD
SA174750CT10TCDD
SA174751CT9TCDD
SA174752CT2TCDD
SA174753CT7TCDD
SA174754CT1TCDD
SA174755CV5Vehicle
SA174756CV4Vehicle
SA174757CV3Vehicle
SA174758CV2Vehicle
SA174759CV6Vehicle
SA174760CV7Vehicle
SA174761CV10Vehicle
SA174762CV9Vehicle
SA174763CV8Vehicle
SA174764CV1Vehicle
Showing results 1 to 20 of 20

Collection:

Collection ID:CO001941
Collection Summary:Six-week-old female wildtype (WT) C57BL/6 mice acquired from Jackson Laboratories (Bar Harbor, ME) were housed in an AAALAC-accredited specific-pathogen-free animal facility located at the grounds of the University of South Carolina School of Medicine for the entirety of all experiments. Mice within the facility were housed within polycarbonate cages containing cellulose fiber chips as bedding in a temperature and humidity-controlled environment. After a 2-week acclimatization period, the mice were divided randomly into two groups that would be administered a single 100µl intraperitoneal injection containing sterile corn oil (VEH group) or an intraperitoneal injection of 10µg/kg TCDD suspended within sterile corn oil (TCDD group). At the 72h time point following TCDD or VEH exposure, the mice were humanely euthanized by an overdose of inhaled isoflurane. Cecal content and blood sera were collected at the time of necropsy.
Sample Type:Cecal content

Treatment:

Treatment ID:TR001960
Treatment Summary:Six-week-old female wildtype (WT) C57BL/6 mice acquired from Jackson Laboratories (Bar Harbor, ME) were housed in an AAALAC-accredited specific-pathogen-free animal facility located at the grounds of the University of South Carolina School of Medicine for the entirety of all experiments. Mice within the facility were housed within polycarbonate cages containing cellulose fiber chips as bedding in a temperature and humidity-controlled environment. After a 2-week acclimatization period, the mice were divided randomly into two groups that would be administered a single 100µl intraperitoneal injection containing sterile corn oil (VEH group) or an intraperitoneal injection of 10µg/kg TCDD suspended within sterile corn oil (TCDD group). At the 72h time point following TCDD or VEH exposure, the mice were humanely euthanized by an overdose of inhaled isoflurane. Cecal content and blood sera were collected at the time of necropsy.

Sample Preparation:

Sampleprep ID:SP001954
Sampleprep Summary:Cecal content and serum samples were processed according to previously described methods for the metabolomic profiling using liquid chromatography-mass spectrometry (LC-MS) with slight modifications. For cecal content, ~25 mg was aliquoted into 1.5-mL Eppendorf tubes (Hamburg, Germany) containing ~20 mg acid washed glass beads (Sigma-Aldrich, St. Louis, MO), extracted into ice-cold MeOH:water (1:1, v/v) on a Qiagen TissueLyzer at 50 Hz for 10 min (Hilden, Germany), and centrifuged at 12,000 rpm for 10 min. For blood sera, 20 µL aliquots were extracted by adding 180 µL cold MeOH, briefly vortexed, and incubated at -20ºC for 30 min for protein precipitation. The supernatants of both matrices were dried in a CentriVap vacuum concentrator (Labconco, MO) and resuspended in ACN:water (2:98, v/v) upon analysis.

Combined analysis:

Analysis ID AN003033
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode POSITIVE
Units m/z

Chromatography:

Chromatography ID:CH002246
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002820
Analysis ID:AN003033
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:fullscan *.raw data acquired by Thermo XCalibur software
Ion Mode:POSITIVE
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