Summary of Study ST001882

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001186. The data can be accessed directly via it's Project DOI: 10.21228/M8DD61 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001882
Study TitleLC-MS for Predator Cues Target Signaling Pathways in Toxic Algal Metabolome Protocol
Study SummaryMetabolomics investigation of the phytoplankton Alexandrium minutum with and without copepod cues in order to explore cell signaling involved in toxin induction.
Institute
Georgia Institute of Technology
Last NameBrown
First NameEmily
Address950 Atlantic Drive, Atlanta GA 30332
Emailemily.brown@gatech.edu
Phone404-894-8424
Submit Date2021-07-15
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-07-27
Release Version1
Emily Brown Emily Brown
https://dx.doi.org/10.21228/M8DD61
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001186
Project DOI:doi: 10.21228/M8DD61
Project Title:Predator Cues Target Signaling Pathways in Toxic Algal Metabolome
Project Type:Metabolomics to elucidate signaling pathway
Project Summary:Early detection of predators is critical to the survival of all living organisms. For phytoplankton, recognition and response to chemical cues from predators, as evidence of predation risk, is particularly crucial. The phytoplankton Alexandrium minutum upregulates its toxicity when exposed to copepodamides, a suite of compounds released by copepod predators. However, how A. minutum perceives these predatory cues and what metabolic pathways are involved in initiating toxin induction remains unknown. In this study LC/MS and NMR-based metabolomics uncovered subtle physiological responses of A. minutum to copepodamides, including dysregulation of valine biosynthesis and enhancement of butanoate metabolism and arginine biosynthesis. While we have yet to identify a chemoreceptor directly activated by copepod cues, based on the results of inhibition experiments detection of copepodamides appears to disrupt the activity of serine/threonine phosphatases leading to increased jasmonic acid biosynthesis and signaling, which leads to amplified gonyautoxin biosynthesis in A. minutum. This study is an important step toward a better understanding of chemosensory ecology of predator-prey interactions in phytoplankton.
Institute:Georgia Institute of Technology
Department:School of Biological Sciences, School of Chemistry and Biochemistry, Center for Microbial Dynamics and Infection, Parker H. Petit Institute for Bioengineering and Bioscience
Laboratory:Kubanek Lab
Last Name:Brown
First Name:Emily
Address:950 Atlantic Dr Atlanta, GA, 30332, USA
Email:julia.kubanek@biosci.gatech.edu
Phone:404-894-8424
Project Comments:This study has 3 parts: 2 NMR (polar and non-polar metabolites) and MS
Contributors:Emily R. Brown, Sam G. Moore, David A. Gaul, and Julia Kubanek

Subject:

Subject ID:SU001959
Subject Type:Other organism
Subject Species:Alexandrium minutum

Factors:

Subject type: Other organism; Subject species: Alexandrium minutum (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA175001Aq_COP_08Copepodamide exposure
SA175002Aq_COP_09Copepodamide exposure
SA175003Aq_COP_10Copepodamide exposure
SA175004Aq_COP_07Copepodamide exposure
SA175005Aq_COP_04Copepodamide exposure
SA175006Aq_COP_02Copepodamide exposure
SA175007Aq_COP_03Copepodamide exposure
SA175008Aq_COP_11Copepodamide exposure
SA175009Aq_COP_06Copepodamide exposure
SA175010Aq_COP_13Copepodamide exposure
SA175011Aq_COP_18Copepodamide exposure
SA175012Aq_COP_19Copepodamide exposure
SA175013Aq_COP_20Copepodamide exposure
SA175014Aq_COP_17Copepodamide exposure
SA175015Aq_COP_16Copepodamide exposure
SA175016Aq_COP_01Copepodamide exposure
SA175017Aq_COP_14Copepodamide exposure
SA175018Aq_COP_15Copepodamide exposure
SA175019Aq_COP_12Copepodamide exposure
SA175020Aq_COP_05Copepodamide exposure
SA175030Aq_Control_05no exposure
SA175031Aq_Control_04no exposure
SA175032Aq_Control_02no exposure
SA175033Aq_Control_06no exposure
SA175034Aq_Control_03no exposure
SA175035Aq_Control_07no exposure
SA175036Aq_Control_16no exposure
SA175037Aq_Control_15no exposure
SA175038Aq_Control_17no exposure
SA175039Aq_Control_01no exposure
SA175040Aq_Control_19no exposure
SA175041Aq_Control_18no exposure
SA175042Aq_Control_14no exposure
SA175043Aq_Control_13no exposure
SA175044Aq_Control_09no exposure
SA175045Aq_Control_08no exposure
SA175046Aq_Control_10no exposure
SA175047Aq_Control_11no exposure
SA175048Aq_Control_12no exposure
SA175049Aq_Control_20no exposure
SA175021Quality_Control_02QC
SA175022Quality_Control_01QC
SA175023Blank_SB_06QC
SA175024Blank_SB_05QC
SA175025Quality_Control_04QC
SA175026Quality_Control_03QC
SA175027Quality_Control_06QC
SA175028Quality_Control_07QC
SA175029Quality_Control_05QC
Showing results 1 to 49 of 49

Collection:

Collection ID:CO001952
Collection Summary:From culture (alexandrium minutum strain CCMP 113)
Sample Type:Algae

Treatment:

Treatment ID:TR001971
Treatment Summary:Copepodamide que

Sample Preparation:

Sampleprep ID:SP001965
Sampleprep Summary:Modified Bligh Dyer

Combined analysis:

Analysis ID AN003042 AN003043 AN003044 AN003045
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish Thermo Vanquish Thermo Vanquish
Column Waters Acquity BEH HILIC (150 x 2.1mm,1.7um) Waters Acquity BEH HILIC (150 x 2.1mm,1.7um) Thermo Accucore C30 (150 x 2.1mm,2.6um) Thermo Accucore C30 (150 x 2.1mm,2.6um)
MS Type ESI ESI ESI ESI
MS instrument type Orbritrap/ion trap Orbritrap/ion trap Orbitrap Orbitrap
MS instrument name Thermo Orbitrap ID-X Tribrid Thermo Orbitrap ID-X Tribrid Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units peak area peak area peak area peak area

Chromatography:

Chromatography ID:CH002255
Chromatography Summary:Polar HILIC separation
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH HILIC (150 x 2.1mm,1.7um)
Chromatography Type:HILIC
  
Chromatography ID:CH002256
Chromatography Summary:non-Polar reverse phase separation
Instrument Name:Thermo Vanquish
Column Name:Thermo Accucore C30 (150 x 2.1mm,2.6um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002830
Analysis ID:AN003042
Instrument Name:Thermo Orbitrap ID-X Tribrid
Instrument Type:Orbritrap/ion trap
MS Type:ESI
MS Comments:Compound Discoverer
Ion Mode:POSITIVE
  
MS ID:MS002831
Analysis ID:AN003043
Instrument Name:Thermo Orbitrap ID-X Tribrid
Instrument Type:Orbritrap/ion trap
MS Type:ESI
MS Comments:Compound Discoverer
Ion Mode:NEGATIVE
  
MS ID:MS002832
Analysis ID:AN003044
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Compound Discoverer
Ion Mode:POSITIVE
  
MS ID:MS002833
Analysis ID:AN003045
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Compound Discoverer
Ion Mode:NEGATIVE
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