Summary of Study ST001911

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001205. The data can be accessed directly via it's Project DOI: 10.21228/M8Z700 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001911
Study TitleA high-fat diet induced obesity murine model treated with bitter melon (Momordica charantia)
Study TypeA 40-day Intervention experiment
Study SummaryMetabolic effects of lyophilized bitter melon juice (BMJ) extract (oral gavage 200mg/kg/body weight-daily for 40 days) intake were evaluated in diet-induced obese C57BL/6J male mice [fed-high fat diet (HFD), 60 kcal% fat]. Changes in metabolite abundance levels in lipid-phase plasma [liquid chromatography mass spectrometry (LC-MS)-based metabolomics] after BMJ intervention were assessed.
Institute
University of Colorado Anschutz Medical Campus
DepartmentPharmaceutical Sciences
LaboratoryDr. Rajesh Agarwal's Laboratory
Last NameAgarwal
First NameRajesh
Address12850 E. Montview Blvd, C238, Room V20-2118, Aurora, CO 80045, USA
EmailRajesh.Agarwal@cuanschutz.edu
Phone303-724-4055
Submit Date2021-05-22
Num Groups2
Total Subjects8
Num Males8
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2021-08-30
Release Version1
Rajesh Agarwal Rajesh Agarwal
https://dx.doi.org/10.21228/M8Z700
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001205
Project DOI:doi: 10.21228/M8Z700
Project Title:Metabolome changes induced by bitter melon (Momordica charantia)- intake in a high-fat diet induced obesity model
Project Type:LCMS quanitative analysis
Project Summary:Quanitative analysis of metabolomics on samples from murine samples (KEGG Pathway MAPS, LIPIDMAPS, Human Metabolome Database)
Institute:University of Colorado Anschutz Medical Campus
Department:Pharmaceutical Sciences
Laboratory:Dr. Rajesh Agarwal's Laboratory and
Last Name:Agarwal
First Name:Rajesh
Address:12850 E. Montview Blvd, C238, Room V20-2118, Aurora, CO 80045, USA
Email:Rajesh.Agarwal@cuanschutz.edu
Phone:303-724-4055
Funding Source:This work was supported by the National Institutes of Health/ National Cancer Institute grant R01CA195708, the National Institutes of Health Office of Dietary Supplements supplemental funds R01CA195708-02S1, and the pilot funding from the office of the Associate Dean for Research and Graduate Education (ADR), School of Pharmacy, UC AMC (to RA), and the National Cancer Institute diversity supplement grant R01CA195708-04S1 (to DR and RA).
Contributors:Dominique Reed, Dileep Kumar, Sushil Kumar, Komal Raina, Reenu Punia, Rama Kant, Laura Saba, Charmion Cruickshank-Quinn, Boris Tabakoff, Nichole Reisdorph, Michael G. Edwards, Michael Wempe, Chapla Agarwal, Rajesh Agarwal

Subject:

Subject ID:SU001989
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:wildtype
Age Or Age Range:4 weeks
Gender:Male
Animal Animal Supplier:Jackson Laboratory
Animal Housing:UC Denver
Animal Feed:High fat diet (HFD), 60 kcal% fat from Research diets Inc., # D12492
Animal Water:ad libitum

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA176929OB_07(raw)High Fat
SA176930OB_12_A(raw)High Fat
SA176931OB_11(raw)High Fat
SA176932OB+BMJ_05(raw)Treated
SA176933OB+BMJ_03(raw)Treated
SA176934OB+BMJ_04(raw)Treated
Showing results 1 to 6 of 6

Collection:

Collection ID:CO001982
Collection Summary:Blood was collected and plasma/serum flash frozen in liquid nitrogen and stored in -80 degrees Celsius for biochemical/metabolic assessments.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR002001
Treatment Summary:Sixteen-week-old diet-induced obese (DIO) C57BL/6J male mice [fed-high fat diet (HFD), 60 kcal% fat from Research diets Inc., # D12492) from 4 weeks of age] were purchased from Jackson labs (Bar Harbor, ME). Once received at the animal house facility in UC Denver, the mice were randomized into two groups (n=4/ group): a) Control-DIO group maintained on the 60 kcal% HFD for additional 40 days, and b) BMJ+DIO group maintained on 60 kcal% HFD fat for additional 40 days plus orally gavage with lyophilized bitter melon juice extract [(5mg per mouse; total volume of 100 μl, 200mg/kg body weight/ day), reconstituted in sterile deionized water immediately before use)] throughout the study.
Treatment:Intervention
Treatment Compound:Bitter melon juice
Treatment Route:Oral gavage
Treatment Dose:200mg/kg body weight/ day
Animal Fasting:On day 40 of study initiation, mice in control and treament groups were subjected to fasting for six hours prior to sacrifice.
Animal Endp Euthanasia:On day 40, mice were sacrificed after CO2 asphyxiation and exsanguination.

Sample Preparation:

Sampleprep ID:SP001995
Sampleprep Summary:Frozen plasma was stored at -80°C until sample preparation for untargeted liquid-chromatography mass spectrometry (LC-MS)-based metabolomics. Briefly, 100µL of sample underwent protein precipitation using methanol, followed by liquid-liquid extraction using methyl-tert butyl ether as previously described[1, 2] to obtain an aqueous fraction and a lipid fraction. The lipid fraction was dried down and resuspended in 100% methanol for LC-MS analysis.
Processing Storage Conditions:Described in summary

Combined analysis:

Analysis ID AN003108
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290
Column Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type TOF
MS instrument name Agilent 6210 TOF
Ion Mode POSITIVE
Units Abundance (mass spectral counts)

Chromatography:

Chromatography ID:CH002295
Chromatography Summary:The samples from the lipid fraction were analyzed an Agilent 1290 series pump with an Agilent Zorbax Rapid Resolution HD (RRHD) SB-C18, 1.8 micron (2.1 x 100 mm) analytical column and an Agilent Zorbax SB-C18, 1.8 micron (2.1 x 5 mm) guard column. The autosampler tray temperature was set at 4°C, column temperature was set at 60°C, and the sample injection volume was 4µL. The flow rate was 0.7 mL/min with the following mobile phases: mobile phase A was water with 0.1% formic acid, and mobile phase B was 60:36:4 isopropyl alcohol:acetonitrile:water with 0.1% formic acid. Gradient elution was as follows: 0-1 minute 30-70% B, 1-7.92 minutes 70-100% B, 7.92-10.4 minutes 100% B, 10.4-10.5 minutes 100-30% B, followed by column re-equilibration with 30% B from 10.5-15.1 minutes.
Instrument Name:Agilent 1290
Column Name:Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um)
Column Temperature:60
Flow Gradient:0-1 minute 30-70% B, 1-7.92 minutes 70-100% B, 7.92-10.4 minutes 100% B, 10.4-10.5 minutes 100-30% B, followed by column re-equilibration with 30% B from 10.5-15.1 minutes.
Flow Rate:0.7 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:60% isopropanol/36% acetonitrile/4% water; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS002890
Analysis ID:AN003108
Instrument Name:Agilent 6210 TOF
Instrument Type:TOF
MS Type:ESI
MS Comments:The mass spectrometry conditions were as follows: Agilent 6210 Time-of-Flight mass spectrometer (TOF-MS) in positive ionization mode with dual electrospray (ESI) source, mass range 60-1600m/z, scan rate 2.03, gas temperature 300°C, gas flow 12.0L/min, nebulizer 30psi, skimmer 60V, capillary voltage 4000V, fragmentor 120V, reference masses 121.050873 and 922.009798 (Agilent reference mix). Proprietary analytical software for integration and peak picking
Ion Mode:POSITIVE
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