Summary of Study ST002022

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001284. The data can be accessed directly via it's Project DOI: 10.21228/M8R71H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002022
Study TitleA Sentinel Serum Quality Management Program for NMR Metabolomics
Study TypePharmacometabolomics
Study SummaryPooled human serum (Sentinel Serum Samples) was aliquoted for storage at -80C and subsequently had NMR spectra acquired annually for three years. This was to determine sentinel serum sample utility for a quality management program to monitor NMR reproducibility. Sentinel serum quality control samples allowed the quantification of variance between different profilers within a project. They also demonstrated declines in metabolite concentrations over time. A sentinel program is useful for tracking changes in QC serum samples from long-term storage. Separately, QC samples show negligible variance introduced by multiple profilers of NMR spectra within a project.
Institute
University of Michigan
DepartmentClinical Pharmacy
LaboratoryUMich NMR Metabolomics Laboratory
Last NameStringer
First NameKathleen
Address428 Church Street
Emailstringek@med.umich.edu; stringek@umich.edu
Phone7346474775
Submit Date2021-11-30
Num Groups3
Total Subjects1
Study CommentsSamples were aliquots of a batch of pooled human serum, collected from multiple different individuals
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailNMR
Release Date2022-01-06
Release Version1
Kathleen Stringer Kathleen Stringer
https://dx.doi.org/10.21228/M8R71H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001284
Project DOI:doi: 10.21228/M8R71H
Project Title:A Sentinel Serum Quality Management Program for NMR Metabolomics
Project Type:Metabolomics
Project Summary:Pooled human serum (Sentinel Serum Samples) was aliquoted for storage at -80C and subsequently had NMR spectra acquired annually for three years. This was to determine sentinel serum sample utility for a quality management program to monitor NMR reproducibility. Sentinel serum quality control samples allowed the quantification of variance between different profilers within a project. They also demonstrated declines in metabolite concentrations over time. A sentinel program is useful for tracking changes in QC serum samples from long-term storage. Separately, QC samples show negligible variance introduced by multiple profilers of NMR spectra within a project.
Institute:University of Michigan
Department:Clinical Pharmacy
Laboratory:UMich NMR Metabolomics Laboratory
Last Name:Stringer
First Name:Kathleen
Address:428 Church Street
Email:stringek@med.umich.edu; stringek@umich.edu
Phone:7346474775
Funding Source:NIH/NIGMS
Contributors:T. Flott, K Stringer

Subject:

Subject ID:SU002104
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Year
SA189410236_0011
SA189411236_0101
SA189412236_0121
SA189413236_0081
SA189414236_0111
SA189415236_0091
SA189416236_0031
SA189417236_0071
SA189418236_0041
SA189419236_0021
SA189420236_0051
SA189421236_0061
SA189422236_0212
SA189423236_0202
SA189424236_0232
SA189425236_0242
SA189426236_0222
SA189427236_0192
SA189428236_0142
SA189429236_0182
SA189430236_0152
SA189431236_0132
SA189432236_0172
SA189433236_0162
SA189434236_0333
SA189435236_0343
SA189436236_0353
SA189437236_0323
SA189438236_0363
SA189439236_0283
SA189440236_0263
SA189441236_0253
SA189442236_0273
SA189443236_0293
SA189444236_0303
SA189445236_0313
Showing results 1 to 36 of 36

Collection:

Collection ID:CO002097
Collection Summary:Samples were aliquoted from pooled sera collected from multiple individuals. Samples were purchased from Innovative Research (Novi, MI).
Sample Type:Blood (serum)
Storage Conditions:-80℃
Storage Vials:Wheaton 2mL Cryovials
Additives:Na Heparin

Treatment:

Treatment ID:TR002116
Treatment Summary:Samples were not treated, beyond the initial aliquotting of the pooled human sera upon receipt at our laboratory before storage at -80C

Sample Preparation:

Sampleprep ID:SP002110
Sampleprep Summary:Prior to spectral acquisition samples were methanol precipitated and ultrafiltered to remove macromolecules prior to spectra acquisition
Sampleprep Protocol Filename:Serum_Precipitation_SOP.docx, Serum_Filtration_SOP.docx
Processing Storage Conditions:On ice
Extraction Method:Methanol Precipitation
Extract Storage:-80℃
Sample Resuspension:Samples were resuspended in 50mM phosphate buffer in D2O
Sample Spiking:Samples were spiked with a Formate solution in D2O of known concentration

Analysis:

Analysis ID:AN003292
Analysis Type:NMR
Num Factors:3
Num Metabolites:31
Units:uM

NMR:

NMR ID:NM000226
Analysis ID:AN003292
Instrument Name:Varian 500 MHz
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:500
NMR Solvent:D2O
NMR Tube Size:5mm
Shimming Method:Auto shim (gradient shimming), with manual shimming when necessary
Pulse Sequence:1D-NOESY
Water Suppression:Saturation at 80Hz induced field strength
Temperature:25
Number Of Scans:32
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