Summary of Study ST002266

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001448. The data can be accessed directly via it's Project DOI: 10.21228/M8JQ4M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002266
Study TitleKīlauea lava fuels phytoplankton bloom in the North Pacific Ocean - study of particulate metabolites
Study TypeStudy of particulate metabolites in phytoplankton blooms.
Study SummaryThese data are relative concentrations of targeted metabolites measured in particulate matter collected from near the Island of Hawai’i in July 2018 during the Kilauea eruption. Six stations were sampled, with station 2 representing the most geothermally impacted station closest to the lava entry. Station 6 was the most oligotrophic station. The particulate metabolites show evidence of altered community composition and metabolism at the geothermally impacted station. This station was within a phytoplankton bloom. The bloom was stimulated by lava heating deep seawater and driving upwelling, which then provided nutrients for diatom growth. See Wilson and Hawco, et. al. 2019 (DOI: 10.1126/science.aax4767) for a complete description of sample collection, phytoplankton bloom dynamics, and chemical modifications of seawater due to the eruption. Metabolites with high concentration (relative abundance per L of seawater) in the geothermally impacted station (St 2) compared to the other stations were: cytosine, hydroxyectoine, adenine, adenosine, thymine, glutamic acid, ectoine, deoxyadenosine, UDP-glucosamine, and guanosine. After normalizing to the particulate carbon concentration at each station, guanosine, glutamic acid, hydroxyectoine, ectoine, adenosine, deoxyadenosine, and UDP-glucosamine were enriched in the geothermally impacted station relative to other stations. Ectoine was the metabolite with the largest change between St 2 and St 6, regardless of normalization to L of seawater or to moles of particulate carbon, with dramatically higher concentrations in the geothermally impacted waters. Except for glutamic and proline, particulate amino acids were generally in higher concentrations in the oligotrophic station.
Institute
University of Washington
DepartmentSchool of Oceanography
LaboratoryIngalls Lab
Last NameLionheart
First NameRegina
Address1400 NE Campus Parkway, Seattle, Washington, 98195, USA
Emailregina16@uw.edu
Phone2062216750
Submit Date2022-08-10
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-09-05
Release Version1
Regina Lionheart Regina Lionheart
https://dx.doi.org/10.21228/M8JQ4M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001448
Project DOI:doi: 10.21228/M8JQ4M
Project Title:Kīlauea lava fuels phytoplankton bloom in the North Pacific Ocean - study of particulate metabolites
Project Type:Marine Metabolomics
Project Summary:From June to August 2018, the eruption of the Kīlauea volcano on the island of Hawai‘i injected millions of cubic meters of molten lava into the nutrient-poor waters of the North Pacific Subtropical Gyre. The lava-impacted seawater was characterized by high concentrations of metals and nutrients that stimulated phytoplankton growth, resulting in an extensive plume of chlorophyll a that was detectable by satellite. Samples for particulate metabolites were collected from different stations surrounding the lava flowing into the ocean to see how marine microorganisms respond to exogenous inputs of nutrients and metals.
Institute:University of Washington
Department:School of Oceanography
Laboratory:Ingalls Lab
Last Name:Lionheart
First Name:Regina
Address:1400 NE Campus Parkway, Seattle, Washington, 98195, USA
Email:regina16@uw.edu
Phone:2062216750
Funding Source:Simons Foundation
Publications:Wilson et al., Science September 2019

Subject:

Subject ID:SU002352
Subject Type:Water sample

Factors:

Subject type: Water sample; Subject species: - (Factor headings shown in green)

mb_sample_id local_sample_id Sampling_Date Latitude Longitude Station Cast Depth sample_vol_filtered_L
SA217503Smp_S2C2D5_A7/13/2018 19.415 154.8416667 S2 C2 5 10
SA217504Smp_S2C2D5_C7/13/2018 19.415 154.8416667 S2 C2 5 10
SA217505Smp_S2C2D5_B7/13/2018 19.415 154.8416667 S2 C2 5 10
SA217506Smp_S5C1D5_A7/14/2018 19.09666667 154.505 S5 C1 5 10
SA217507Smp_S5C1D5_B7/14/2018 19.09666667 154.505 S5 C1 5 9
SA217508Smp_S4C2D5_B7/14/2018 19.38166667 154.6766667 S4 C2 5 10
SA217509Smp_S4C2D5_C7/14/2018 19.38166667 154.6766667 S4 C2 5 10
SA217510Smp_S4C2D5_A7/14/2018 19.38166667 154.6766667 S4 C2 5 10
SA217511Smp_S3TF_A7/14/2018 19.435 154.7933333 S3 TF 1 10
SA217512Smp_S6C3D5_B7/15/2018 18.695 154.5233333 S6 C3 5 10
SA217513Smp_S6C3D5_A7/15/2018 18.695 154.5233333 S6 C3 5 10
SA217514Smp_S6C3D5_C7/15/2018 18.695 154.5233333 S6 C3 5 9
SA217515Poo_HOT-LAVA-QC_37/15/2018 18.74166667 155.3816667 QC Underway from ship 5 20
SA217516Poo_HOT-LAVA-QC_17/15/2018 18.74166667 155.3816667 QC Underway from ship 5 20
SA217517Poo_HOT-LAVA-QC_27/15/2018 18.74166667 155.3816667 QC Underway from ship 5 20
SA217518Poo_TruePoo_Half3NA NA NA NA NA NA NA
SA217519Smp_KM1513Poo7-26_1NA NA NA NA NA NA NA
SA217520Smp_S515mMS_BNA NA NA NA NA NA NA
SA217521Smp_S515mMS_CNA NA NA NA NA NA NA
SA217522Poo_TruePoo_Half2NA NA NA NA NA NA NA
SA217523Smp_S515mMS_ANA NA NA NA NA NA NA
SA217524Poo_TruePoo_Full3NA NA NA NA NA NA NA
SA217525Poo_TruePoo_DDApos20NA NA NA NA NA NA NA
SA217526Blk_MQBlk_1NA NA NA NA NA NA NA
SA217527Poo_TruePoo_DDApos35NA NA NA NA NA NA NA
SA217528Poo_TruePoo_DDApos50NA NA NA NA NA NA NA
SA217529Poo_TruePoo_Full2NA NA NA NA NA NA NA
SA217530Poo_TruePoo_Full1NA NA NA NA NA NA NA
SA217531Poo_TruePoo_Half1NA NA NA NA NA NA NA
Showing results 1 to 29 of 29

Collection:

Collection ID:CO002345
Collection Summary:Samples for particulate metabolites were collected from 5 different stations surrounding the lava flowing into the ocean off the island of Hawaii, all from a depth of 5m. At each sampling location, single, duplicate, or triplicate filters were collected using either niskins attached to a conductivity, temperature, depth array (CTD) or the underway intake. Samples (10 L) were collected into polycarbonate carboys, filtered onto 147 mm 0.2 μm PTFE filters using peristaltic pumps, polycarbonate filter holders, and Masterflex PharMed BPT tubing (Cole-Parmer). Filters were flash frozen in liquid nitrogen and stored at -80°C until extraction. In addition to our samples, we filtered MilliQ water through a 0.2 μm PTFE filter and extracted this filter alongside samples as a methodological blank.
Sample Type:Phytoplankton
Collection Method:CTD Niskin arrays
Collection Location:Hawai'i
Volumeoramount Collected:10L
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002364
Treatment Summary:Samples (10 L) were collected into polycarbonate carboys, filtered onto 147 mm 0.2 μm PTFE filters using peristaltic pumps, polycarbonate filter holders, and Masterflex PharMed BPT tubing (Cole-Parmer). Filters were flash frozen in liquid nitrogen and stored at -80°C until extraction.

Sample Preparation:

Sampleprep ID:SP002358
Sampleprep Summary:Each sample was extracted using a modified Bligh-Dyer extraction. Briefly, filters were cut up and put into 15 mL teflon centrifuge tubes containing a mixture of 100 µm and 400 µm silica beads. Heavy isotope-labeled internal standards were added along with ~2 mL of cold aqueous solvent (50:50 methanol:water) and ~3 mL of cold organic solvent (dichloromethane). The samples were shaken on a FastPrep-24 Homogenizer for 30 seconds and chilled in a -20 °C freezer repeatedly for three cycles of bead-beating and a total of 30 minutes of chilling. The organic and aqueous layers were separated by spinning samples in a centrifuge at 4,300 rpm for 2 minutes at 4 °C. The aqueous layer was removed to a new glass centrifuge tube. The remaining organic fraction was rinsed three more times with additions of 1 to 2 mL of 50:50 methanol:water. All aqueous rinses were combined for each sample and ~2 mL of cold dichloromethane was added to the combined aqueous layer. Tubes were shaken and centrifuged at 4,300 rpm for 2 minutes at 4°C. The aqueous layer was removed to a new glass vial and dried under N2 gas. The remaining organic layer in the bead beating tubes was transferred into the glass centrifuge tube and the bead beating tube was rinsed two more times with cold organic solvent. The combined organic rinses were centrifuged, transferred to a new glass vial, and dried under N2 gas. Dried aqueous fractions were re-dissolved in 380 µL of water. Dried organic fractions were re-dissolved in 400 µL of 90:10 methanol:toluene. 20 µL of isotope-labeled injection standards in water were added to the aqueous fractions. Blank filters were extracted alongside samples as methodological blanks.

Combined analysis:

Analysis ID AN003701 AN003702 AN003703
Analysis type MS MS MS
Chromatography type HILIC HILIC GC
Chromatography system Q Exactive™ Plus Hybrid Quadrupole-Orbitrap Q Exactive™ Plus Hybrid Quadrupole-Orbitrap Q Exactive™ Plus Hybrid Quadrupole-Orbitrap
Column SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um) Waters Acquity UPLC HSS Cyano (100 x 2.1mm,1.8um)
MS Type ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE
Units Normalized peak area Normalized peak area Normalized peak area

Chromatography:

Chromatography ID:CH002742
Chromatography Summary:See attached summary
Methods Filename:Ingalls_Metabolomics_LC_HOT-LAVA.txt
Instrument Name:Q Exactive™ Plus Hybrid Quadrupole-Orbitrap
Column Name:SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
Column Temperature:30
Flow Rate:0.15 ml/min
Solvent A:85% acetonitrile/15% water; 10 mM ammonium carbonate
Solvent B:15% acetonitrile/85% water; 10 mM ammonium carbonate
Chromatography Type:HILIC
  
Chromatography ID:CH002743
Chromatography Summary:See attached summary
Methods Filename:Ingalls_Metabolomics_LC_HOT-LAVA.txt
Instrument Name:Q Exactive™ Plus Hybrid Quadrupole-Orbitrap
Column Name:Waters Acquity UPLC HSS Cyano (100 x 2.1mm,1.8um)
Chromatography Type:GC

MS:

MS ID:MS003451
Analysis ID:AN003701
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See protocol
Ion Mode:POSITIVE
Analysis Protocol File:Ingalls_Metabolomics_MS_HOT-LAVA.txt
  
MS ID:MS003452
Analysis ID:AN003702
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See protocol
Ion Mode:NEGATIVE
Analysis Protocol File:Ingalls_Metabolomics_MS_HOT-LAVA.txt
  
MS ID:MS003453
Analysis ID:AN003703
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See protocol
Ion Mode:POSITIVE
Analysis Protocol File:Ingalls_Metabolomics_MS_HOT-LAVA.txt
  logo